Human CellExp™ LRRC4 /NGL-2, human recombinant

Biomolecule/Target: LRRC4 /NGL-2

Synonyms: LRRC4, NGL-2, BAG, NAG14.

Alternates names: LRRC4, NGL-2, BAG, NAG14.

Taglines: Regulates the formation of excitatory synapses through the recruitment of pre-and-postsynaptic proteins

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 64101

NCBI Gene Symbol: LRRC4

Gene Source: Human

Accession #: Q9HBW1

Recombinant: Yes

Source: HEK293 cells

Purity by SDS-PAGE #: ≥92%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: <1 EU/μg by LAL method

Activity (Specifications/test method): N/A

Biological activity: Measured by its binding ability in a functional ELISA. Immobilized Recombinant mouse Netrin-G2a Fc chimera at 4 μg/ml, can bind rhLRRC4 with an apparent KD < 10 nM.

Results: Measured by its binding ability in a functional ELISA. Immobilized Recombinant mouse Netrin-G2a Fc chimera at 4 μg/ml, can bind rhLRRC4 with an apparent KD < 10 nM.

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: This protein is fused with 6×His tag at the C-terminus, has a calculated MW of 55.6 kDa. The predicted N-terminus is Ala 39. DTT-reduced Protein migrates as 90-110 kDa due to glycosylation.

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Background Information: LRRC4 (Leu-rich repeat/LRR-containing glycoprotein 4), LRRC4B and LRRC4C are post-synaptic adhesion molecules of the LRR protein family that induce excitatory synapse formation. LRRC4 is also known as Brain tumor-associated protein BAG, Netrin-G2 ligand (NGL-2), Nasopharyngeal carcinoma-associated gene 14 protein (NAG14), which contains 1 Ig-like (immunoglobulin-like) domain and 9 LRR (leucine-rich) repeats, 1 LRRCT domain, 1 LRRNT domain. LRRC4 / NGL-2 specifically expressed in brain. LRRC4 / NGL-2 regulates the formation of excitatory synapses through the recruitment of pre-and-postsynaptic proteins. LRRC4 / NGL-2 organize the lamina/pathway-specific differentiation of dendrites. LRRC4 / NGL-2 plays an important role for auditory synaptic responses and involved in the suppression of glioma.

Amino acid sequence: N/A

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans