Human CellExp™ Nectin-2 / CD112, Human Recombinant

Biomolecule/Target: NECTIN2

Synonyms: CD112, Nectin-2, PVRL2

Alternates names: CD112, Nectin-2, PVRL2

Taglines: Modulator of T-cell signaling.

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 5819

NCBI Gene Symbol: NECTIN2

Gene Source: Human

Accession #: Q92692

Recombinant: Yes

Source: HEK 293 cells

Purity by SDS-PAGE #: ≥95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: < 1.0 EU per 1 μg of protein

Activity (Specifications/test method): Measured by its binding ability in a functional ELISA

Biological activity: Immobilized Human PVRIG, Mouse IgG2a Fc Tag, low endotoxin at 2 μg/mL (100 μL/well) can bind Human Nectin-2, His Tag with a linear range of 0.078-0.625 μg/mL

Results: N/A

Binding Capacity: Immobilized Human PVRIG, Mouse IgG2a Fc Tag, low endotoxin at 2 μg/mL (100 μL/well) can bind Human Nectin-2, His Tag with a linear range of 0.078-0.625 μg/mL

Unit Definition: N/A

Molecular Weight: 37.4 kDa

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from 0.22 μm filtered solution in PBS, pH5.0. Normally trehalose is added as protectant before lyophilization.

Reconstitution Instructions: Reconstitute in sterile deionized water to a concentration of 100 µg/ml.

Background Information: Poliovirus receptor-related 2 (PVRL2) is also known as nectin-2 and CD112 (formerly herpesvirus entry mediator B, HVEB), which is a human plasma membrane glycoprotein. PVRL2 is one of the plasma membrane components of adherens junctions. PVRL2 also serves as an entry for certain mutant strains of herpes simplex virus and pseudorabies virus, and it is involved in cell to cell spreading of these viruses. Variations in PVRL2 gene have been associated with differences in the severity of multiple sclerosis. Also, PVRL2 binds with low affinity to TIGIT. Furthermore, PVRL2 acts as a receptor for herpes simplex virus 1 (HHV-1) mutant Rid1, herpes simplex virus 1 (HHV-2) and pseudorabies virus (PRV).

Amino acid sequence: N/A

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans