Human CellExp™ PD-1 /PDCD1, C-Fc Tag, mouse recombinant

Biomolecule/Target: PD-1/PDCD1

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Alternates names: PDCD1, PD1, CD279, SLEB2, hPD-1, hPD-l

Taglines: Inhibits the T-cell proliferation and production of IL-1, IL-4, IL-10 and IFN-γ by suppressing the activation and transduction of PI3K/AKT pathway.

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 18566

NCBI Gene Symbol: PDCD1

Gene Source: Mouse

Accession #: Q02242

Recombinant: Yes

Source: HEK293 cells

Purity by SDS-PAGE #: ≥92%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: <1 EU/μg by LAL method

Activity (Specifications/test method): N/A

Biological activity: Measured by its binding ability in a functional ELISA. Immobilized recombinant mouse PD-1 /PDCD1 Fc Chimera at 2 µg/mL (100 µl/well) can bind biotinylated Mouse PD-L1 with a linear range of 32 - 500 ng/mL.

Results: Measured by its binding ability in a functional ELISA. Immobilized recombinant mouse PD-1 /PDCD1 Fc Chimera at 2 µg/mL (100 µl/well) can bind biotinylated Mouse PD-L1 with a linear range of 32 - 500 ng/mL.

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: This protein is fused with a human IgG1 Fc tag at the C-terminus, and has a calculated MW of 43.2 kDa. The predicted N-terminus is Ser 21. DTT-reduced Protein migrates as 60-66 kDa in SDS-PAGE due to glycosylation.

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM glycine, pH 7.5. Normally Mannitol or Trehalose is added as protectants before lyophilization.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Background Information: Programmed cell death protein 1 (PD-1) is also known as CD279 and PDCD1, is a type I membrane protein and is a member of the extended CD28/CTLA-4 family of T cell regulators. PDCD1 is expressed on the surface of activated T cells, B cells, macrophages, myeloid cells and a subset of thymocytes. PD-1 has two ligands, PD-L1 and PD-L2, which are members of the B7 family. PD-L1 is expressed on almost all murine tumor cell lines, including PA1 myeloma, P815 mastocytoma, and B16 melanoma upon treatment with IFN-γ. PD-L2 expression is more restricted and is expressed mainly by DCs and a few tumor lines. PD1 inhibits the T-cell proliferation and production of related cytokines including IL-1, IL-4, IL-10 and IFN-γ by suppressing the activation and transduction of PI3K/AKT pathway. In addition, coligation of PD1 inhibits BCR-mediating signal by dephosphorylating key signal transducer. In vitro, treatment of anti-CD3 stimulated T cells with PD-L1-Ig results in reduced T cell proliferation and IFN-γ secretion. Monoclonal antibodies targeting PD-1 that boost the immune system are being developed for the treatment of cancer. This protein is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. It can also be used as an immunogen, as a protein standard, or in cell biology research applications.

Amino acid sequence: N/A

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans