Human CellExp™ SCF, Human Recombinant

Biomolecule/Target: N/A

Synonyms: Human Cellexp Human Recombinant SCF

Alternates names: Kit ligand Precursor, C-kit ligand, SCF, Mast cell growth factor, MGF, SF, KL-1, Kit l, DKFZp686F2250.

Taglines: A cytokine playing an important role in hematopoiesis, spermatogenesis, & melanogenesis

Taglines: USA

Country of Animal Origin: China

NCBI Gene ID #.: 4254

NCBI Gene Symbol: KITLG

Gene Source: Human

Accession #: P21583

Recombinant: Yes

Source: Human 293 cell expressed

Purity by SDS-PAGE #: > 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: < 1.0 EU per 1 μg of protein

Activity (Specifications/test method): N/A

Biological activity: ED₅₀ is typically 5 to 25 ng/mL. The specific activity was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line).

Results: 5 to 25 ng/ml

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 35 to 45 kDa, monomer, glycosylated

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized in PBS.

Reconstitution Instructions: Reconstitute in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.

Background Information: Stem cell factor / KIT ligand (SCF) is a cytokine which binds CD117(c-Kit). SCF is also known as "steel factor" or "c-kit ligand". SCF exists in two forms; cell surface bound SCF and soluble (or free) SCF. Soluble SCF is produced by the cleavage of surface bound SCF by metalloproteases. SCF is a growth factor important for the survival, proliferation, and differentiation of hematopoietic stem cells and other hematopoietic progenitor cells. One of its roles is to change the BFU-E (burst-forming unit-erythroid) cells, which are the earliest erythrocyte precursors in the erythrocytic series, into the CFU-E (colony-forming unit-erythroid).

Amino acid sequence: N/A

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans