Human CellExp™ Thrombomodulin, Human Recombinant
- Usually Shipped in 5 Working Days
- Storage Temperature:
- Shipping Conditions:
- Gel Pack
- Shelf Life:
- 12 months
Synonyms: CD141, BDCA-3, THBD, TM
Alternates names: CD141, BDCA-3, THBD, TM
Taglines: An endothelial cell expressed transmembrane glycoprotein
Country of Animal Origin: USA
NCBI Gene ID #.: 7056
NCBI Gene Symbol: THBD
Gene Source: Human
Accession #: P07204
Source: HEK 293 cells
Purity by SDS-PAGE #: ≥98%
Assay #2: HPLC
Endotoxin Level: < 0.1 ng/μg of protein (<1EU/μg).
Activity (Specifications/test method): Measured by its ability to activate protein C induced cleavage of the chromogenic substrate, BOC-Asp-Pro Arg-AMC in the presence of thrombin. The specific activity is >500 pmoles/min/µg.
Biological activity: Measured by its ability to activate protein C induced cleavage of the chromogenic substrate, BOC-Asp-Pro Arg-AMC in the presence of thrombin. The specific activity is >500 pmoles/min/µg.
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: 51.4 kDa
Appearance: Lyophilized powder
Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM Sodium phosphate, pH 7.5.
Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Background Information: Thrombomodulin (TM, CD141, and THBD) is an endothelial cell expressed transmembrane glycoprotein that can form a complex with the coagulation factor, Thrombin. The Thrombomodulin/Thrombin complex converts protein C to its activated form, protein Ca, which in turn proteolytically cleaves and deactivates factor Va and factor VIIIa, two essential components of the coagulation mechanism. This inactivation reduces the generation of additional thrombin and thereby effectively prevents continued coagulation. Reduced levels of Thrombomodulin can correlate with the pathogenesis of certain cardiovascular diseases, such as atherosclerosis and thrombosis. However, the serum levels of the truncated circulating form of Thrombomodulin are typically elevated during inflammation and in the presence of various inflammatory related diseases. Recombinant soluble Thrombomodulin is a 491 amino acid glycoprotein containing the extracellular domain of Thrombomodulin.
Amino acid sequence: APAEPQPGGS QCVEHDCFAL YPGPATFLNA SQICDGLRGH LMTVRSSVAA DVISLLLNGD GGVGRRRLWI GLQLPPGCGD PKRLGPLRGF QWVTGDNNTS YSRWARLDLN GAPLCGPLCV AVSAAEATVP SEPIWEEQQC EVKADGFLCE FHFPATCRPL AVEPGAAAAA VSITYGTPFA ARGADFQALP VGSSAAVAPL GLQLMCTAPP GAVQGHWARE APGAWDCSVE NGGCEHACNA IPGAPRCQCP AGAALQADGR SCTASATQSC NDLCEHFCVP NPDQPGSYSC MCETGYRLAA DQHRCEDVDD CILEPSPCPQ RCVNTQGGFE CHCYPNYDLV DGECVEPVDP CFRANCEYQC QPLNQTSYLC VCAEGFAPIP HEPHRCQMFC NQTACPADCD PNTQASCECP EGYILDDGFI CTDIDECENG GFCSGVCHNL PGTFECICGP DSALARHIGT DCDSGKVDGG DSGSGEPPPS PTPGSTLTPP A
Handling: Centrifuge the vial prior to opening.
Usage: For Research Use Only! Not to be used in humans