Human CellExp™ sDLL-1, Human Recombinant
- Usually Shipped in 5 Working Days
- Storage Temperature:
- Shipping Conditions:
- Gel Pack
- Shelf Life:
- 12 months
Synonyms: Delta-like protein 1, Delta-1
Alternates names: Delta-like protein 1, Delta-1
Taglines: Activates the Notch-1 and Notch-2 receptors
Country of Animal Origin: USA
NCBI Gene ID #.: 28514
NCBI Gene Symbol: DLL-1
Gene Source: Human
Accession #: O00548
Source: HEK 293 cells
Purity by SDS-PAGE #: ≥95%
Assay #2: HPLC
Endotoxin Level: < 0.1 ng/μg of protein (<1EU/μg).
Activity (Specifications/test method): Determined by the dose dependent growth suppression of the human acute monocytic leukemia cell line, THP-1. sDLL-1 inhibits the proliferation in THP-1 cells using a concentration of 3.0-5.0 μg/ml.
Biological activity: Determined by the dose dependent growth suppression of the human acute monocytic leukemia cell line, THP-1. sDLL-1 inhibits the proliferation in THP-1 cells using a concentration of 3.0-5.0 μg/ml.
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: 57-60 kDa
Appearance: Lyophilized powder
Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized from 1X PBS, pH 7.5.
Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Background Information: Human sDLL-1 comprises the extracellular signaling domain of DLL-1, a member of a structurally-related family of single-pass type I trans-membrane proteins that serve as ligands for Notch receptors. It is expressed in the heart and pancreas, and to a lesser extent in various other tissues. DLL-1 functions to specifically activate the Notch-1 and Notch-2 receptors. The Notch signaling pathway regulates endothelial-cell differentiation, proliferation and apoptosis, and is essential for the development, maintenance and remodeling of the vascular system. DLL-1 suppresses differentiation of hematopoietic progenitor cells into the B-cell lineage while promoting differentiation to T-cell and NK cell precursors. Recombinant human sDLL-1 is a 57.0-60.0 kDa glycoprotein containing 522 amino-acid residues.
Amino acid sequence: SGVFELKLQE FVNKKGLLGN RNCCRGGAGP PPCACRTFFR VCLKHYQASV SPEPPCTYGS AVTPVLGVDS FSLPDGGGAD SAFSNPIRFP FGFTWPGTFS LIIEALHTDS PDDLATENPE RLISRLATQR HLTVGEEWSQ DLHSSGRTDL KYSYRFVCDE HYYGEGCSVF CRPRDDAFGH FTCGERGEKV CNPGWKGPYC TEPICLPGCD EQHGFCDKPG ECKCRVGWQG RYCDECIRYP GCLHGTCQQP WQCNCQEGWG GLFCNQDLNY CTHHKPCKNG ATCTNTGQGS YTCSCRPGYT GATCELGIDE CDPSPCKNGG SCTDLENSYS CTCPPGFYGK ICELSAMTCA DGPCFNGGRC SDSPDGGYSC RCPVGYSGFN CEKKIDYCSS SPCSNGAKCV DLGDAYLCRC QAGFSGRHCD DNVDDCASSP CANGGTCRDG VNDFSCTCPP GYTGRNCSAP VSRCEHAPCH NGATCHERGH RYVCECARGY GGPNCQFLLP ELPPGPAVVD LTEKLEGQGG PF
Handling: Centrifuge the vial prior to opening.
Usage: For Research Use Only! Not to be used in humans