Human CellExp™ VEGF R1 /Flt-1, Rhesus macaque Recombinant

Biomolecule/Target: N/A

Synonyms: FLT, VEGFR1, FLT1

Alternates names: FLT, VEGFR1, FLT1

Taglines: Plays an essential role in the development of embryonic vasculature

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 574133

NCBI Gene Symbol: FLT1

Gene Source: Rhesus macaque

Accession #: F7FAY2

Recombinant: TRUE

Source: HEK 293 cells

Purity by SDS-PAGE #: >95% as determined by SDS-PAGE

Assay: N/A

Purity: N/A

Assay #2: N/A

Endotoxin Level: Less than 0.1 EU per μg by the LAL method.

Activity (Specifications/test method): Immobilized Rhesus macaque PLGF at 0.5 μg/mL (100 μL/well) can bind Rhesus macaque VEGF R1, Mouse IgG2a Fc Tag with a linear range of 0.6-10 ng/mL. Immobilized Rhesus macaque VEGF R1, Mouse IgG2a Fc Tag, at 2 μg/mL (100 μL/well) can bind Biotinylated Human VEGF165, His Tag with a linear range of 0.5-3.9 ng/mL

Biological activity: N/A

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: The protein has a calculated MW of 109.1 kDa. The protein migrates as 130 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Concentration: N/A

Appearance: Lyophilized powder

Physical form description: Lyophilized

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile deionized water to a concentration of 100 µg/ml. Do not vortex. It is recommended to store at -20°C.

Background Information: Vascular endothelial growth factor receptor 1 (VEGFR1) is also known as Fms-like tyrosine kinase 1 (FLT-1), Tyrosine-protein kinase receptor FLT, is a single-pass type I membrane protein and secreted protein which belongs to the protein kinase superfamily, Tyr protein kinase family and CSF-1/PDGF receptor subfamily. VEGFR1 is detected in normal lung, but also in placenta, liver, kidney, heart and brain tissues and specifically expressed in most of the vascular endothelial cells, and also expressed in peripheral blood monocytes. VEGFR1 acts as a cell-surface receptor for VEGFA, VEGFB and PGF, and plays an essential role in the development of embryonic vasculature, the regulation of angiogenesis, cell survival, cell migration, macrophage function, chemotaxis, and cancer cell invasion. VEGFR1 may play an essential role as a negative regulator of embryonic angiogenesis by inhibiting excessive proliferation of endothelial cells. VEGFR1 can promote endothelial cell proliferation, survival and angiogenesis in adulthood.

Amino acid sequence: AA Ser 27 - Asn 756

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans