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CaspSELECT™ Caspase-3 Immunoassay Kit (Fluorometric)

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SKU:
26-K163
Availability:
Usually Shipped in 5 Working Days
Size:
100 Assays
Storage Conditions:
-20°C
Shipping Conditions:
Gel Pack
Shelf life:
6-12 months
€798.00
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Description

Activation of caspase-3 plays a key role in initiation of cellular events during apoptosis. The CaspSELECT™ Caspase-3 Immunoassay Kit provides an effective immunosorbent enzyme assay for specific, quantitative detection of caspase-3 activity in microtiter plates. The assay utilizes caspase-3 polyclonal antibody to capture activated caspase-3 from cell lysates. Caspase substrate DEVD-AFC is then added and is cleaved proportionally to the amount of activated caspase-3 in the cell lysate. The cleavage generates free AFC which can be analyzed fluorometrically (Ex./Em. = 400 nm/505 nm) using a fluorescence plate reader. The assay ensures absolute specific detection of caspase-3. Other known caspases and non-specific proteases are not detected.

Alternate Name: N/A

Tag Line: Specifically detects Caspase-3

Summary: • Detection method- Fluorescence (Ex/Em 370-425/ 490-525 nm) • Species reactivity- Mammalian • Kit size- 100 assays • Applications- Effective immunosorbent enzyme assay for specific, quantitative detection of caspase-3 activity in microtiter plates.

Detection Method: N/A

Sample Type: Cell and tissue lysates

Species Reactivity: N/A

Applications: N/A

Features & Benefits: • Simple one-step procedure; takes only ~3 hours • Fast and convenient

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