CaspSELECT™ Caspase-3 Immunoassay Kit (Fluorometric)
- Usually Shipped in 5 Working Days
- 100 Assays
- Storage Conditions:
- Shipping Conditions:
- Gel Pack
- Shelf life:
- 6-12 months
Activation of caspase-3 plays a key role in initiation of cellular events during apoptosis. The CaspSELECT™ Caspase-3 Immunoassay Kit provides an effective immunosorbent enzyme assay for specific, quantitative detection of caspase-3 activity in microtiter plates. The assay utilizes caspase-3 polyclonal antibody to capture activated caspase-3 from cell lysates. Caspase substrate DEVD-AFC is then added and is cleaved proportionally to the amount of activated caspase-3 in the cell lysate. The cleavage generates free AFC which can be analyzed fluorometrically (Ex./Em. = 400 nm/505 nm) using a fluorescence plate reader. The assay ensures absolute specific detection of caspase-3. Other known caspases and non-specific proteases are not detected.
Alternate Name: N/A
Tag Line: Specifically detects Caspase-3
Summary: • Detection method- Fluorescence (Ex/Em 370-425/ 490-525 nm) • Species reactivity- Mammalian • Kit size- 100 assays • Applications- Effective immunosorbent enzyme assay for specific, quantitative detection of caspase-3 activity in microtiter plates.
Detection Method: N/A
Sample Type: Cell and tissue lysates
Species Reactivity: N/A
Features & Benefits: • Simple one-step procedure; takes only ~3 hours • Fast and convenient