Biovision
BioSim™ Vedolizumab (Human) ELISA Kit
- SKU:
- 26-E4697
- Availability:
- Usually Shipped in 5 Working Days
- Size:
- 96 Assays
- Storage Conditions:
- 4°C
- Shipping Conditions:
- Gel Pack
- Shelf life:
- 12 months
Description
Vedolizumab is a recombinant humanized IgG1 monoclonal antibody directed against the human lymphocyte α4β7 integrin, a key mediator of gastrointestinal inflammation. It is used in the treatment of moderate to severe active ulcerative colitis and Crohn's disease for patients who have had an inadequate response with, lost response to, or were intolerant to inhibitors of tumor necrosis factor-alpha (TNF-alpha) or other conventional therapies. BioSim™ Vedolizumab ELISA kit is a Solid-phase enzyme-linked immunosorbent assay (ELISA) based on the sandwich principle. Standards and samples (serum or plasma) are incubated in the microtitre plate coated with the reactant for Vedolizumab. After incubation, the wells are washed. A horseradish peroxidase (HRP) conjugated probe is added and binds to Vedolizumab captured by the reactant on the surface of the wells. Following incubation, wells are washed and the bound enzymatic activity is detected by the addition of chromogen-substrate. The color developed is proportional to the amount of Vedolizumab in the sample or standard. The test results can be deduced directly from the standard curve.
Alternate Name: Vedolizumab (Entyvio®)(Human) ELISA Kit,Entyvio ELISA Kit,Vedolizumab ELISA Kit
Tag Line: This ELISA kit is used for in vitro quantitative determination of Vedolizumab.
Summary: Vedolizumab is a recombinant humanized IgG1 monoclonal antibody directed against the human lymphocyte α4β7 integrin, a key mediator of gastrointestinal inflammation. It is used in the treatment of moderate to severe active ulcerative colitis and Crohn's disease for patients who have had an inadequate response with, lost response to, or were intolerant to inhibitors of tumor necrosis factor-alpha (TNF-alpha) or other conventional therapies. BioSim™ Vedolizumab ELISA kit is a Solid phase enzyme-linked immunosorbent assay (ELISA) based on the sandwich principle. Standards and samples (serum or plasma) are incubated in the microtitre plate coated with the reactant for Vedolizumab (Cyramza®). After incubation, the wells are washed. A horse radish peroxidase (HRP) conjugated probe is added and binds to Vedolizumab captured by the reactant on the surface of the wells. Following incubation wells are washed and the bound enzymatic activity is detected by addition of chromogen-substrate. The color developed is proportional to the amount of Vedolizumab in the sample or standard.
Detection Method: colorimetric
Sample Type: Plasma,Serum
Species Reactivity: Human
Applications: Sensitivity: 30 ng/ml,Recovery rate: 85 – 115% with normal human serum samples with known concentrations,Detection Range: 30 - 1000 ng/ml,This ELISA kit is used for in vitro quantitative determination of Vedolizumab,Cross Reactivity: Except for Vedolizumab, there is no cross reaction with other therapeutic antibodies and native serum immunoglobins,Assay Precision: Intra-Assay: CV < 15%; Inter-Assay: CV < 15% (CV (%) = SD/mean X 100)
Features & Benefits: Detection Range: 3 - 100 ng/ml Sensitivity: 3 ng/ml Cross Reactivity: Except for Vedolizumab, there is no cross reaction with other therapeutic antibodies and native serum immunoglobins. This ELISA kit is used for in vitro quantitative determination of Vedolizumab