Human CellExp™ ADIPOQ /Adiponectin /ACRP30, human recombinant

Biomolecule/Target: Adiponectin

Synonyms: ADIPOQ, Adiponectin, ACDC, ACRP30, APM1, GBP28

Alternates names: ADIPOQ, Adiponectin, ACDC, ACRP30, APM1, GBP28

Taglines: Involved in the control of fat metabolism and insulin sensitivity

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 9370

NCBI Gene Symbol: ADIPOQ

Gene Source: Human

Accession #: Q15848

Recombinant: Yes

Source: HEK 293 cells

Purity by SDS-PAGE #: ≥95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: < 0.1 ng/μg of protein (<1EU/μg).

Activity (Specifications/test method): Measured by its ability to induce TIMP-1 secretion by mouse macrophages. The ED50 for this effect is typically 2 - 10 µg/ml.

Biological activity: N/A

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: The protein is fused with 6×His tag at the C-terminus, has a calculated MW of 25.4 kDa

Concentration: N/A

Appearance: Lyophilized powder

Physical form description: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH7.4.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Background Information: Adiponectin also referred to as GBP-28, APM1, ADIPOQ, ACDC and Acrp30, is a secreted protein which contains one C1q domain (commonly called the globular domain) and one collagen-like domain. Adiponectin / ADIPOQ is synthesized exclusively by adipocytes and secreted into plasma. ADIPOQ is important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Adiponectin antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Acrp30 Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway and may play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW.

Amino acid sequence: N/A

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans