Human CellExp™ Biotinylated PD-L1, Human Recombinant

Biomolecule/Target: N/A

Synonyms: Programmed Cell Death 1 Ligand 1, PDL1, PDCD1L1, CD274, B7 homolog 1, B7H1, B7-H1

Alternates names: Programmed Cell Death 1 Ligand 1, PDL1, PDCD1L1, CD274, B7 homolog 1, B7H1, B7-H1

Taglines: N/A

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 29126

NCBI Gene Symbol: CD274

Gene Source: Human

Accession #: Q9NZQ7

Recombinant: False

Source: HEK293 cells

Purity by SDS-PAGE #: ≥95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): BioVision’s Biotinylated Human Recombinant PD-L1 has been tested for its biotin content (2-3 biotins per PD-L1 as determined by K811-100) and ability to bind Human PD-1 (Cat. No. 7498) in a functional ELISA with a linear range of 2-125 ng (0.02–1.25 μg/ml)

Biological activity: N/A

Results: N/A

Binding Capacity: BioVision’s Biotinylated Human Recombinant PD-L1 has been tested for its biotin content (2-3 biotins per PD-L1 as determined by K811-100) and ability to bind Human PD-1 (Cat. No. 7498) in a functional ELISA with a linear range of 2-125 ng (0.02–1.25 μg/ml)

Unit Definition: N/A

Molecular Weight: The protein is fused with 6×His tag at the N-terminus, has a calculated MW of 26.4 kDa. The protein migrates as 35 -40 kDa in SDS-PAGE under reducing conditions due to glycosylation.

Concentration: 0.5 mg/ml

Appearance: Sterile Filtered colorless solution

Physical form description: Liquid

Reconstitution Instructions: N/A

Background Information: Human Programmed Cell Death 1 Ligand-1 (PD-L1) is also known as CD274 and B7-H1) is a type I transmembrane protein which binds to its receptor, Programmed Cell Death protein 1 (PD-1). PD-L1 is widely distributed on leukocytes, nonhematopoietic cells, and in nonlymphoid tissues including islets. PD-L1 and PD-1 pathway serves as an “immune checkpoint” and its blockade is a highly promising therapy for a broad spectrum of cancers.

Amino acid sequence: Leu 25-Gln 167

Handling: Centrifuge the vial prior to opening.

Usage: N/A