Human CellExp™ Biotinylated CD28, Human Recombinant

Biomolecule/Target: N/A

Synonyms: T-cell-specific surface glycoprotein CD28; CD antigen: TP44; Tp44; T-Cell-Specific Surface Glycoprotein; CD28 Molecule

Alternates names: T-cell-specific surface glycoprotein CD28; CD antigen: TP44; Tp44; T-Cell-Specific Surface Glycoprotein; CD28 Molecule

Taglines: Involved in T-cell activation and survival

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 940

NCBI Gene Symbol: N/A

Gene Source: Human

Accession #: P10747

Recombinant: True

Source: HEK 293 cells

Purity by SDS-PAGE #: > 95% by SDS-PAGE

Assay: N/A

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): Biotinylated human CD28-Fc can bind to immobilized Human CD80, Fc Tag (Cat. No. P1668 coated at 1 µg/mL, 100 µL/well) with a linear range 0.01 – 0.27µg/ml.

Biological activity: N/A

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: ~42 kDa (Human Fc Tag fused to C-terminus, Biotinylated)

Concentration: N/A

Appearance: Lyophilized protein

Physical form description: Lyophilized powder

Reconstitution Instructions: N/A

Background Information: Human CD28 is composed of four exons encoding a protein of 220 amino acids that is expressed on the cell surface as a glycosylated, disulfide-linked homodimer of 44 kDa. Members of the CD28 family share a number of common features. These receptors consist of paired V-set immunoglobulin superfamily (IgSF) domains attached to single transmembrane domains and cytoplasmic domains that contain critical signaling motifs. The CD28 and CTLA4 ligands, CD80 and CD86, consist of single V-set and C1-set IgSF domains. The interaction of these costimulatory receptors with ligands is mediated through the MYPPPY motif within the receptor V-set domains. CD28 is expressed constitutively on almost all human CD4 T cells and approximately 50% of CD8 T cells. CD28 costimulation has diverse effects on T cell function, including biochemical events at the immunological synapse, downstream phosphorylation and other post-translational modifications, transcriptional changes, and cytoskeletal remodeling. At the most basic level, CD28 signals increase a cell’s glycolytic rate, allowing cells to generate the energy necessary for growth and proliferation.

Amino acid sequence: Asn 19 - Pro 152

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans