Human CellExp™ PD-L1 /CD274 /B7-H1, human recombinant

Biomolecule/Target: PD-L1 /CD274 /B7-H1

Synonyms: PD-L1, CD274, B7-H1, PDCD1L1, PDCD1LG1

Alternates names: PD-L1, CD274, B7-H1, PDCD1L1, PDCD1LG1

Taglines: Involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner.

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 29126

NCBI Gene Symbol: CD274

Gene Source: Human

Accession #: Q9NZQ7

Recombinant: Yes

Source: HEK293 cells

Purity by SDS-PAGE #: ?95%

Assay: SDS-PAGE

Purity: ?90%

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): Measured by its binding ability in a functional ELISA

Biological activity: Immobilized Human PD-L1, His Tag at 5 ?g/mL (100 ?L/well) can bind Human PD-1, His Tag with a linear range of 0.15-1.25 ?g/mL.

Results: N/A

Binding Capacity: Immobilized Human PD-L1, His Tag at 5 ?g/mL (100 ?L/well) can bind Human PD-1, His Tag with a linear range of 0.15-1.25 ?g/mL.

Unit Definition: N/A

Molecular Weight: This protein is fused with polyhistidine tag at the C-terminus, has a calculated MW of 26.4 kDa. The predicted N-terminus is Phe 19. DTT-reduced Protein migrates as 35-40 kDa due to glycosylation.

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from 0.22 ?m filtered solution in PBS pH 7.4

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Background Information: Programmed cell death 1 ligand 1 (PD-L1) is also known as cluster of differentiation (CD274) or B7 homolog 1 (B7-H1). It has been identified as the ligand for PD1/PDCD1 (Cat. No. 7498) and has been demonstrated to play a role in the regulation of immune r

Amino acid sequence: N/A

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans