Biovision
Human CellExp™ Cathepsin D, human recombinant
- SKU:
- 26-7409
- Availability:
- Usually Shipped in 5 Working Days
- Size:
- 10 μg
- Storage Temperature:
- -20°C
- Shipping Conditions:
- Gel Pack
- Shelf Life:
- 12 months
Description
Biomolecule/Target: Cathepsin D
Synonyms: CTSD, Cathepsin D, CPSD, CLN10
Alternates names: CTSD, Cathepsin D, CPSD, CLN10
Taglines: Involved in protein degradation in lysosomes
Taglines: USA
Country of Animal Origin: USA
NCBI Gene ID #.: 1509
NCBI Gene Symbol: CTSD
Gene Source: Human
Accession #: P07339
Recombinant: Yes
Source: HEK293 cells
Purity by SDS-PAGE #: ≥95%
Assay: SDS-PAGE
Purity: N/A
Assay #2: N/A
Endotoxin Level: <1 EU/μg by LAL method
Activity (Specifications/test method): N/A
Biological activity: Measured by its ability to cleave the fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH2. The specific activity is >450 pmol/min/µg.
Results: Measured by its ability to cleave the fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH2. The specific activity is >450 pmol/min/µg.
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: This protein is fused with polyhistidine tag at the C-terminus, has a calculated MW of 43.6 kDa. The predicted N-terminus is Ser 19. DTT-reduced Protein migrates as 45-55 kDa due to glycosylation.
Concentration: N/A
Appearance: Lyophilized
Physical form description: Lyophilized from 0.22 μm filtered solution in 50 mM MES, pH 6.5 with 100 mM NaCl. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.
Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Background Information: Cathepsin D is also known as CTSD, CPSD, which belongs to the peptidase A1 family. Cathepsin D can be cleaved into the following 2 chains: cathepsin D light chain and cathepsin D heavy chain, which is expressed in the aorta extracellular space (at protein level). The catalytic activity of Cathepsin D is specificity similar to, but narrower than, that of pepsin A. Cathepsin D does not cleave the 4-Gln-|-His-5 bond in B chain of insulin. Cathepsin D involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease.
Amino acid sequence: N/A
Handling: Centrifuge the vial prior to opening.
Usage: For Research Use Only! Not to be used in humans