Human CellExp™ CD244 / 2B4 / SLAMF4, human recombinant

Biomolecule/Target: CD244/2B4/SLAMF4

Synonyms: CD244, 2B4, SLAMF4, NKR2B4, NAIL, h2B4

Alternates names: CD244, 2B4, SLAMF4, NKR2B4, NAIL, h2B4

Taglines: Modulates receptor-ligand interactions to enhance leukocyte activation

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 51744

NCBI Gene Symbol: CD244

Gene Source: Human

Accession #: Q9BZW8

Recombinant: Yes

Source: HEK293 cells

Purity by SDS-PAGE #: ≥95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: <1 EU/μg by LAL method

Activity (Specifications/test method): N/A

Biological activity: Measured by its binding ability in a functional ELISA. Immobilized rh CD244 / 2B4 at 2 μg/ml (100 μl/well) can bind human CD48. The EC50 of human CD48 is 0.39 μg/ml.

Results: Measured by its binding ability in a functional ELISA. Immobilized rh CD244 / 2B4 at 2 μg/ml (100 μl/well) can bind human CD48. The EC50 of human CD48 is 0.39 μg/ml.

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: This protein is fused with polyhistidine tag at the C-terminus, and has a calculated MW of 23.1 kDa. The predicted N-terminus is Cys 22. DTT-reduced Protein migrates as 40-50 kDa due to glycosylation.

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Background Information: Natural killer cell receptor 2B4 is also known as NK cell type I receptor protein 2B4 (NKR2B4 or h2B4), SLAM family member 4 (SLAMF4), Signaling lymphocytic activation molecule 4, CD antigen CD244. NKR2B4 / CD244 contain two Ig-like (immunoglobulin-like) domains. CD244 is expressed in spleen, PBL, followed by lung, liver, testis and small intestine. CD244 interacts with CD48. Following phosphorylation, CD244 is able to recruit PTPN11/SHP-2 and SH2D1A/SAP. SLAMF4 modulate other receptor-ligand interactions to enhance leukocyte activation. CD244/2B4 is the only heterophilic receptor of SLAM family.

Amino acid sequence: N/A

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans