Human CellExp™ CD3 epsilon & CD3 delta Heterodimer
- Usually Shipped in 5 Working Days
- 10 μg
- Storage Temperature:
- Shipping Conditions:
- Gel Pack
- Shelf Life:
- 12 months
Biomolecule/Target: CD3E, CD3D
Synonyms: CD3E & CD3D, CD3 delta & CD3 epsilon, CD3 delta / epsilon
Alternates names: CD3E & CD3D, CD3 delta & CD3 epsilon, CD3 delta / epsilon
Taglines: T cell receptor plays an important role in coupling antigen recognition
Country of Animal Origin: USA
NCBI Gene ID #.: 916, 915
NCBI Gene Symbol: CD3E, CD3D
Gene Source: Human
Accession #: P07766, P04234
Source: Expi293 cells
Purity by SDS-PAGE #: >95%
Assay #2: N/A
Endotoxin Level: < 1.0 EU per/μg
Activity (Specifications/test method): Measured by its binding ability in a functional ELISA.
Biological activity: Human CD3 epsilon & CD3 delta Heterodimer Protein was immobilized at 0.5 µg/ml (100 µl/well). Dose response curve using Anti-hCD3E/CD3D Ab foralumab gave an EC₅₀ of 21.69 ng/ml by ELISA. Human CD3 epsilon & CD3 delta Heterodimer Protein was Immobilized at 0.1 µg/ml (100 µl/Well). Dose response curve using Anti-hCD3E/CD3D Ab OKT3 gave an EC₅₀ of 21 ng/ml by ELISA.
Binding Capacity: Immobilized OKT3 MAb at 5 μg/mL (100 μL/well) can bind Human CD3E & CD3D, His Tag with a linear range of 0.625-5 μg/mL
Unit Definition: N/A
Molecular Weight: 16.7 kDa and 13.5 kDa
Physical form description: Lyophilized from 0.22 μm filtered solution in PBS pH 7.4. Generally 5-8% Mannitol or Trehalose is added as a protectant before lyophilization.
Reconstitution Instructions: Reconstitute in sterile deionized water to the desired protein concentration.
Background Information: T-cell surface glycoprotein CD3 delta & CD3 epsilon chain, also known as CD3D & CD3E or CD3D&CD3E respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon (CD3E), CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.
Amino acid sequence: CD3E (Asp 23-Asp 126) and CD3D (Phe 22-Ala 105) (Fc tag at C-terminal)
Handling: Centrifuge the vial prior to opening.
Usage: For Research Use Only! Not to be used in humans