Human CellExp™ CD30 /TNFRSF8, human recombinant

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SKU:
26-7389
Availability:
Usually Shipped in 5 Working Days
Size:
50 μg
Storage Temperature:
-20°C
Shipping Conditions:
Gel Pack
Shelf Life:
12 months
£673.38
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Description

Biomolecule/Target: CD30 /TNFRSF8

Synonyms: TNFRSF8, CD30, D1S166E, Ki-1

Alternates names: TNFRSF8, CD30, D1S166E, Ki-1

Taglines: May play a role in the regulation of cellular growth and transformation of activated lymphoblasts

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 943

NCBI Gene Symbol: TNFRSF8

Gene Source: Human

Accession #: P28908

Recombinant: Yes

Source: HEK293 cells

Purity by SDS-PAGE #: ≥95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: <1 EU/μg by LAL method

Activity (Specifications/test method): N/A

Biological activity: Measured by its ability to block CD30 Ligand-induced IL-6 secretion by HDLM human Hodgkin’s lymphoma cells. The ED50 for this effect is typically 0.5 - 2.5 µg/ml in the presence of 50 ng/ml of Recombinant Human CD30 Ligand.

Results: Measured by its ability to block CD30 Ligand-induced IL-6 secretion by HDLM human Hodgkin’s lymphoma cells. The ED50 for this effect is typically 0.5 - 2.5 µg/ml in the presence of 50 ng/ml of Recombinant Human CD30 Ligand.

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: This protein is fused with 6×His tag at the C-terminus, has a calculated MW of 39.3 kDa. The predicted N-terminus is Phe 19. DTT-reduced Protein migrates as 75-90 kDa due to glycosylation.

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Background Information: Human CD30, also known as TNFRSF8, is a cell membrane protein of the tumor necrosis factor receptor family and tumor marker. TNFRSF-8 is expressed by activated, but not by resting, T and B cells. Also, CD30 is expressed on classical Hodgkin Lymphoma cells together with CD15. CD30 is the receptor for TNFSF8/CD30L. CD30 can interact with TRAF2 and TRAF5, and mediate the signal transduction that leads to the activation of NF-kappa-B. TNFRSF8 may play a role in the regulation of cellular growth and transformation of activated lymphoblasts. TNFRSF8 is a positive regulator of apoptosis, and also has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity.

Amino acid sequence: N/A

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans

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