Human CellExp™ CD306 / LAIR2, human recombinant

Biomolecule/Target: CD306/LAIR2

Synonyms: LAIR2, CD306

Alternates names: LAIR2, CD306

Taglines: Plays a potential role in modulating mucosal tolerance

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 3904

NCBI Gene Symbol: LAIR2

Gene Source: Human

Accession #: Q6ISS4

Recombinant: Yes

Source: HEK293 cells

Purity by SDS-PAGE #: ≥95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: <1 EU/μg by LAL method

Activity (Specifications/test method): N/A

Biological activity: Measured by the ability of the immobilized protein to support the adhesion of HT-29 human colon adenocarcinoma cells. When 10000 cells/well are added to rhLAIR2 /CD306 coated plates (50 μg/ml with 100 μl/well), approximately >40% cells will adhere after 10 minutes at 37°C

Results: Measured by the ability of the immobilized protein to support the adhesion of HT-29 human colon adenocarcinoma cells. When 10000 cells/well are added to rhLAIR2 /CD306 coated plates (50 μg/ml with 100 μl/well), approximately >40% cells will adhere after 10 minutes at 37°C

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: This protein is fused with 6×His tag at the C-terminus, has a calculated MW of 14.9 kDa. The predicted N-terminus is Gln 22. DTT-reduced Protein migrates as 19-23 kDa.

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Background Information: Leukocyte-associated immunoglobulin-like receptor 2 (LAIR2) is also known as CD antigen CD306. LAIR2 contains one Ig-like C2-type (immunoglobulin-like) domain. LAIR2 is a member of the immunoglobulin superfamily and was identified by its similarity to LAIR1, an inhibitory receptor present on mononuclear leukocytes. LAIR2 / CD306 is thought to be secreted and may help modulate mucosal tolerance.

Amino acid sequence: N/A

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans