Human CellExp™ CD48 / BCM1 / SLAMF2, human recombinant

Biomolecule/Target: CD48/BCM1/SLAMF2

Synonyms: CD48, BCM1, SLAMF2, BLAST1, BLAST, BLAST1, MEM-102, TCT.1, BCM-1, SLAMF-2, BLAST-1

Alternates names: CD48, BCM1, SLAMF2, BLAST1, BLAST, BLAST1, MEM-102, TCT.1, BCM-1, SLAMF-2, BLAST-1

Taglines: Facilitates interaction between activated lymphocytes

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 962

NCBI Gene Symbol: CD48

Gene Source: Human

Accession #: P09326

Recombinant: Yes

Source: HEK293 cells

Purity by SDS-PAGE #: ≥95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: <1 EU/μg by LAL method

Activity (Specifications/test method): N/A

Biological activity: Measured by its binding ability in a functional ELISA. Immobilized human CD244 protein Fc Chimera at 5 μg/ml (100 μl/well) can bind human CD48 with a linear range of 0.1 - 4 μg/ml.

Results: Measured by its binding ability in a functional ELISA. Immobilized human CD244 protein Fc Chimera at 5 μg/ml (100 μl/well) can bind human CD48 with a linear range of 0.1 - 4 μg/ml.

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: This protein is fused with 6×His tag at the C-terminus, has a calculated MW of 23.2 kDa. The predicted N-terminus is Gln 27. DTT-reduced Protein migrates as 38-45 kDa due to glycosylation.

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Background Information: CD48 antigen is also known as Signaling lymphocytic activation molecule 2, SLAM family member 2 (SLAMF2), Leukocyte antigen MEM-102, BCM1 surface antigen, B-lymphocyte activation marker BLAST-1. CD48 is a 65 kDa GPI-linked protein which belongs to the CD2 subfamily of immunoglobulin superfamily molecules. CD48 antigen contains two Ig-like C2-type (immunoglobulin-like) domains and two Ig-like V-type (immunoglobulin-like) domain. CD48 interacts with CD244 in an heterophilic manner. CD48 is ligand for CD2. CD48 might facilitate interaction between activated lymphocytes. CD48 is probably involved in regulating T-cell activation.

Amino acid sequence: N/A

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans