Human CellExp™ CD74/DHLAG, human recombinant

Biomolecule/Target: CD74/DHLAG

Synonyms: CD74, DHLAG, HLADG, II, Ia-GAMMA, p33, CLIP, INVG34

Alternates names: CD74, DHLAG, HLADG, II, Ia-GAMMA, p33, CLIP, INVG34

Taglines: Plays a critical role in MHC class II antigen processing

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 972

NCBI Gene Symbol: CD74

Gene Source: Human

Accession #: P04233

Recombinant: Yes

Source: HEK293 cells

Purity by SDS-PAGE #: ≥95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: <1 EU/μg by LAL method

Activity (Specifications/test method): N/A

Biological activity: Measured by its binding ability in a functional ELISA. Immobilized human CD74 at 5 μg/ml (100 μl/well) can bind biotinylated human CTSL1 with a linear range of 3.2 - 500 ng/ml.

Results: Measured by its binding ability in a functional ELISA. Immobilized human CD74 at 5 μg/ml (100 μl/well) can bind biotinylated human CTSL1 with a linear range of 3.2 - 500 ng/ml.

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: This protein is fused with 6×His tag at the N-terminus, has a calculated MW of 19.3 kDa. The predicted N-terminus is Gln 73. DTT-reduced Protein migrates as 23-33 kDa due to glycosylation.

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Background Information: Cluster of Differentiation 74 (CD74) also known as HLA class II histocompatibility antigen gamma chain (HLADG), is a polypeptide involved in the formation and transport of MHC class II protein. The cell surface form of the invariant chain is known as CD74. CD74 is upregulated in several cancers and is also expressed by non-immune cells during inflammation. CD74 plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha / beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to the endosomal / lysosomal system where the antigen processing and binding of antigenic peptides to MHC class II takes place. CD74 / DHLAG also serves as cell surface receptor for the cytokine MIF.

Amino acid sequence: N/A

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans