Human CellExp™ CD85J/LILRB1, human recombinant

Biomolecule/Target: CD85J/LILRB1

Synonyms: CD85J, LILRB1, CD85, ILT2, LIR1, MIR7

Alternates names: CD85J, LILRB1, CD85, ILT2, LIR1, MIR7

Taglines: A receptor for class I MHC antigens

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 10859

NCBI Gene Symbol: LILRB1

Gene Source: Human

Accession #: Q8NHL6

Recombinant: Yes

Source: HEK293 cells

Purity by SDS-PAGE #: ≥95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: N/A

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: This protein is fused to the 6x His tag at the C-terminus and has a calculated MW of 49 kDa. The protein migrates as 55-70 kDa under reducing conditions (SDS-PAGE) due to glycosylation.

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in PBS, pH 7.4. Do not vortex.

Background Information: CD85 antigen-like family member J (CD85J) also known as Leukocyte immunoglobulin-like receptor subfamily B member 1 (LILRB1 or LIR-1), Immunoglobulin-like transcript 2 (ILT-2), Monocyte/macrophage immunoglobulin-like receptor 7 (MIR7), which belongs to leukocyte immunoglobulin-like receptor (LIR) family. CD85J / LILRB1 Contains 4 Ig-like C2-type (immunoglobulin-like) domains. CD85J / LIR-1 is expressed predominantly on B-cells and monocytes. CD85J is receptor for class I MHC antigens and recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles and is also receptor for H301/UL18, a human cytomegalovirus class I MHC homolog. Ligand binding results in inhibitory signals and down-regulation of the immune response. CD85J / LILRB1 interaction with HLA-B or HLA-E leads to inhibition of the signal triggered by FCER1A and inhibits serotonin release. CD85J / LILRB1 inhibits FCGR1A-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions.

Amino acid sequence: N/A

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans