Human CellExp™ CD87 / uPAR / PLAUR, human recombinant

Biomolecule/Target: CD87/uPAR/PLAUR

Synonyms: uPAR, PLAUR, CD87, MO3

Alternates names: uPAR, PLAUR, CD87, MO3

Taglines: Acts as a receptor for urokinase plasminogen activator

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 5329

NCBI Gene Symbol: PLAUR

Gene Source: Human

Accession #: Q03405

Recombinant: Yes

Source: HEK293 cells

Purity by SDS-PAGE #: ≥92%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: <1 EU/μg by LAL method

Activity (Specifications/test method): N/A

Biological activity: Measured by its binding ability in a functional ELISA. Immobilized human uPAR at 5 μg/ml (100 μl/well) can bind biotinylated human UPA with a linear range of 25 - 500 ng/ml.

Results: Measured by its binding ability in a functional ELISA. Immobilized human uPAR at 5 μg/ml (100 μl/well) can bind biotinylated human UPA with a linear range of 25 - 500 ng/ml.

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: This protein is fused with polyhistidine tag at the C-terminus, has a calculated MW of 32.1 kDa. The predicted N-terminus is Leu 23. DTT-reduced Protein migrates as 44-48 kDa due to glycosylation.

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Background Information: Urokinase plasminogen activator surface receptor (U-PAR) is also known as PLAUR, Monocyte activation antigen Mo3, CD antigen CD87. PLAUR contains three UPAR/Ly6 domains. U-PAR is expressed in neurons of the rolandic area of the brain (at protein level) and is also expressed in the brain. PLAUR / CD87 interacts with MRC2, SRPX2 and SORL1. PLAUR / UPAR act as a receptor for urokinase plasminogen activator and plays a role in localizing and promoting plasmin formation. U-PAR mediates the proteolysis-independent signal transduction activation effects of U-PA.

Amino acid sequence: N/A

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans