Human CellExp™CEACAM6/CD66c, human recombinant

Biomolecule/Target: CEACAM6/CD66c

Synonyms: CEACAM6, CD66c, CEAL, NCA

Alternates names: CEACAM6, CD66c, CEAL, NCA

Taglines: Affects the sensitivity of tumor cells to adenovirus infection. Widely used as a tumor marker in serum immunoassay determinations of carcinomas.

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 4680

NCBI Gene Symbol: CEACAM6

Gene Source: Human

Accession #: P40199

Recombinant: Yes

Source: HEK 293 cells

Purity by SDS-PAGE #: ≥95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: < 1.0 EU per μg of the rhCEACAM6 by the LAL method.

Activity (Specifications/test method): Measured by the ability of the immobilized protein to support the adhesion of calcium ionophore treated human neutrophils. When 2 x 105 cells/well are added to CEACAM6 coated plates (10 μg/mL, 100 μL/well), 45-70% of the cells will adhere after 20 minutes at 37ºC.

Biological activity: Measured by the ability of the immobilized protein to support the adhesion of calcium ionophore treated human neutrophils. When 2 x 105 cells/well are added to CEACAM6 coated plates (10 μg/mL, 100 μL/well), 45-70% of the cells will adhere after 20 minutes at 37ºC.

Results: Measured by the ability of the immobilized protein to support the adhesion of calcium ionophore treated human neutrophils. When 2 x 105 cells/well are added to CEACAM6 coated plates (10 μg/mL, 100 μL/well), 45-70% of the cells will adhere after 20 minutes at 37ºC.

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: This protein rhCEACAM6 is fused with 6×his tag at C-terminus, has a calculated MW of 32 kDa expressed. The predicted N-terminus is Lys35. Protein migrates as 35-65 kDa in reduced SDS-PAGE resulting from glycosylation.

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in PBS, pH 7.4. Do not vortex.

Background Information: Carcinoembryonic antigen-related cell adhesion molecule 6 (non-specific cross reacting antigen) (CEACAM6) also known as CD66c (Cluster of Differentiation 66c), CEAL, NCA, and is one of seven human CEACAM family members within the immunoglobulin superfamily. In humans, CEACAMs include type I transmembrane proteins (CEACAM1, CEACAM3, and CEACAM4) and GPI-linked molecules (CEACAM5 through CEACAM8). There is no human CEACAM2. CEACAM 6 contains one N-terminal V-type Ig-like domain (N domain), followed by two C2-type Ig-like domains. It shows considerable glycosylation, including (sialyl) LewisX, which mediates binding to E-selectin, galectins and some bacterial fimbrae. CEACAM-6 is expressed by granulocytes and their progenitors. It is also expressed by epithelia of various organs and is upregulated in pancreatic and colon adenocarcinomas, as well as hyperplastic polyps. Resistance to adhesion-related apoptosis in tumor cells is conferred in the condition of CEACAM6 overexpression.

Amino acid sequence: N/A

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans