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Human CellExp™ Complement C5, Human Recombinant

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SKU:
26-P1467
Availability:
Usually Shipped in 5 Working Days
Storage Temperature:
-20°C
Shipping Conditions:
Gel Pack
Shelf Life:
12 months
€185.00 - €536.00
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Description

Biomolecule/Target: N/A

Synonyms: Complement C5, C5, CPAMD4

Alternates names: Complement C5, C5, CPAMD4

Taglines: a potent chemotactic factor for human peripheral blood neutrophils and monocytes

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 718

NCBI Gene Symbol: C3

Gene Source: Human

Accession #: P01031-1

Recombinant: True

Source: HEK 293 cells

Purity by SDS-PAGE #: >90%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Activity (Specifications/test method): N/A

Biological activity: Immobilized Human Complement C5 at 2 μg/mL (100 μL/well) can bind Anti-C5a with a linear range of 0.2-3 ng/mL

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: This protein carries a polyhistidine tag at the C-terminus. The mature form of Complement C5 is a disulfide-linked heterodimer composed of proteolytically cleaved α and β chain. Each α and β chain has a calculated MW of 73.3 kDa (β chain) and 114.4 kDa (α chain). The protein migrates as 66 kDa (β chain) and 110 kDa (α chain) under reducing (R) condition (SDS-PAGE) due to glycosylation.

Concentration: N/A

Appearance: Lyophilized powder

Physical form description: Lyophilized

Reconstitution Instructions: Reconstitute in sterile deionized water to the desired protein concentration.

Background Information: Derived from proteolytic degradation of complement C5, C5 anaphylatoxin is a mediator of local inflammatory process. C5 precursor is first processed by the removal of 4 basic residues, forming two chains, beta and alpha, linked by a disulfide bond. C5 convertase activates C5 by cleaving the alpha chain, releasing C5a anaphylatoxin and generating C5b (beta chain + alpha' chain). Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled. The C5a anaphylatoxin interacts with C5AR1 and tick complement inhibitor. C5a is also a potent chemokine which stimulates the locomotion of polymorphonuclear leukocytes and directs their migration toward sites of inflammation.

Amino acid sequence: Gln 19 - Cys 1676

Handling: Centrifuge the vial prior to opening.

Usage: N/A

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