Biovision
Human CellExp™ Complement C5, Human Recombinant
- SKU:
- 26-P1467
- Availability:
- Usually Shipped in 5 Working Days
- Storage Temperature:
- -20°C
- Shipping Conditions:
- Gel Pack
- Shelf Life:
- 12 months
Description
Biomolecule/Target: N/A
Synonyms: Complement C5, C5, CPAMD4
Alternates names: Complement C5, C5, CPAMD4
Taglines: a potent chemotactic factor for human peripheral blood neutrophils and monocytes
Taglines: USA
Country of Animal Origin: USA
NCBI Gene ID #.: 718
NCBI Gene Symbol: C3
Gene Source: Human
Accession #: P01031-1
Recombinant: True
Source: HEK 293 cells
Purity by SDS-PAGE #: >90%
Assay: SDS-PAGE
Purity: N/A
Assay #2: N/A
Endotoxin Level: Less than 1.0 EU per μg by the LAL method.
Activity (Specifications/test method): N/A
Biological activity: Immobilized Human Complement C5 at 2 μg/mL (100 μL/well) can bind Anti-C5a with a linear range of 0.2-3 ng/mL
Results: N/A
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: This protein carries a polyhistidine tag at the C-terminus. The mature form of Complement C5 is a disulfide-linked heterodimer composed of proteolytically cleaved α and β chain. Each α and β chain has a calculated MW of 73.3 kDa (β chain) and 114.4 kDa (α chain). The protein migrates as 66 kDa (β chain) and 110 kDa (α chain) under reducing (R) condition (SDS-PAGE) due to glycosylation.
Concentration: N/A
Appearance: Lyophilized powder
Physical form description: Lyophilized
Reconstitution Instructions: Reconstitute in sterile deionized water to the desired protein concentration.
Background Information: Derived from proteolytic degradation of complement C5, C5 anaphylatoxin is a mediator of local inflammatory process. C5 precursor is first processed by the removal of 4 basic residues, forming two chains, beta and alpha, linked by a disulfide bond. C5 convertase activates C5 by cleaving the alpha chain, releasing C5a anaphylatoxin and generating C5b (beta chain + alpha' chain). Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled. The C5a anaphylatoxin interacts with C5AR1 and tick complement inhibitor. C5a is also a potent chemokine which stimulates the locomotion of polymorphonuclear leukocytes and directs their migration toward sites of inflammation.
Amino acid sequence: Gln 19 - Cys 1676
Handling: Centrifuge the vial prior to opening.
Usage: N/A