Human CellExp™ CSF1R / CD115 / CD6, human recombinant

Biomolecule/Target: CSF1R / CD115 / CD6

Synonyms: CD6, CD-6, Cluster of Differentiation 6, FLJ44171, TP120

Alternates names: CD6, CD-6, Cluster of Differentiation 6, FLJ44171, TP120

Taglines: ≥98% pure, active, human cell expressed, human recombinant CSF1R / CD115 / CD6, which mediates all effects of cytokine CSF-1

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 1436

NCBI Gene Symbol: CSF1R

Gene Source: Human

Accession #: P07333

Recombinant: Yes

Source: HEK293 cells

Purity by SDS-PAGE #: ≥98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: <1 EU/μg by LAL method

Activity (Specifications/test method): N/A

Biological activity: Measured by its ability to inhibit the MCSF-induced proliferation of NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is typically 1.5-8.7 pg/ml in the presence of 1 ng/ml of rhMCSF.

Results: The ED50 for this effect is typically 1.5-8.7 pg/ml in the presence of 1 ng/ml of rhMCSF.

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: This protein rhCSF1R is fused with a C-terminal 6×his tag and has a calculated MW of 55.1 kDa. The predicted N-terminus is Ile 20. DTT-reduced protein migrates as 80-95 kDa polypeptide in SDS-PAGE.

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in PBS, pH 7.4. Do not vortex.

Background Information: Colony stimulating factor 1 receptor (CSF1R), also known as macrophage colony-stimulating factor receptor (M-CSFR), CD115 Cluster of Differentiation 115 (CD115), C-FMS, CSFR, FIM2, FMS, and is a member of the type III subfamily of receptor tyrosine kinases (RTKs). CSF1R is a receptor for a cytokine called colony stimulating factor 1, The protein encoded by the CSFR1 gene is the receptor for colony stimulating factor 1, a cytokine which controls the production, differentiation, and function of macrophages. This receptor mediates most, if not all, of the biological effects of this cytokine. Ligand binding activates CSFR1 through a process of oligomerization and transphosphorylation. Mutations in CSF1R are associated with chronic myelomonocytic leukemia and type M4 acute myeloblastic leukemia. Increased levels of CSF1R1 are found in microglia in Alzheimer's disease and after brain injuries. The increased receptor expression causes microglia to become more active. Both CSF1R, and its ligand colony stimulating factor 1 play an important role in the development of the mammary gland and may be involved in the process of mammary gland carcinogenesis.

Amino acid sequence: N/A

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans