Human CellExp™ ENPP-2 / Autotaxin, human recombinant

Biomolecule/Target: ENPP-2 / Autotaxin

Synonyms: ENPP2, E-NPP 2, Autotaxin, LysoPLD, ATX, PDNP2, ATX-X, NPP2, PD-IALPHA

Alternates names: ENPP2, E-NPP 2, Autotaxin, LysoPLD, ATX, PDNP2, ATX-X, NPP2, PD-IALPHA

Taglines: ≥92% pure, active, human cell expressed, human recombinant ENPP-2 / Autotaxin - stimulates migration of melanoma cells

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 5168

NCBI Gene Symbol: ENPP2

Gene Source: Human

Accession #: Q13822

Recombinant: Yes

Source: HEK293 cells

Purity by SDS-PAGE #: ≥92%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: <1 EU/μg by LAL method

Activity (Specifications/test method): N/A

Biological activity: Measured by its ability to cleave Bis (p-Nitrophenyl) Phosphate (BPNPP). The specific activity is >8000 pmol/min/µg.

Results: >8000 pmol/min/µg

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: This protein is fused with polyhistidine tag at the N-terminus, has a calculated MW of 94.5 kDa. The predicted N-terminus is Asp 49. DTT-reduced Protein migrates as 120-130 kDa due to glycosylation.

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH 7.5. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in PBS, pH 7.4. Do not vortex.

Background Information: Autotaxin also known as ectonucleotide pyrophosphatase / phosphodiesterase family member 2 (E-NPP 2 or ENPP2), Extracellular lysophospholipase D (LysoPLD), Autotaxin (ATX or ATX-X), which belongs to the nucleotide pyrophosphatase / phosphodiesterase family. ENPP2 contains two SMB (somatomedin-B) domains. ENPP2 hydrolyzes lysophospholipids to produce lysophosphatidic acid (LPA) in extracellular fluids. ENPP2 can act on sphingosylphosphphorylcholine producing sphingosine-1-phosphate, a modulator of cell motility. ENPP2 stimulates migration of melanoma cells, probably via a pertussis toxin-sensitive G protein.

Amino acid sequence: N/A

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans