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- Human CellExp™ ENPP-2 / Autotaxin, human recombinant
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Human CellExp™ ENPP-2 / Autotaxin, human recombinant
- SKU:
- 26-7516
- UPC:
- MPN:
- Availability:
- Usually Shipped in 5 Working Days
- Size:
- 10 μg
- Storage Temperature:
- -20°C
- Shipping Conditions:
- Gel Pack
- Shelf Life:
- 12 months
Description
Biomolecule/Target: ENPP-2 / Autotaxin
Synonyms: ENPP2, E-NPP 2, Autotaxin, LysoPLD, ATX, PDNP2, ATX-X, NPP2, PD-IALPHA
Alternates names: ENPP2, E-NPP 2, Autotaxin, LysoPLD, ATX, PDNP2, ATX-X, NPP2, PD-IALPHA
Taglines: ≥92% pure, active, human cell expressed, human recombinant ENPP-2 / Autotaxin - stimulates migration of melanoma cells
Taglines: USA
Country of Animal Origin: USA
NCBI Gene ID #.: 5168
NCBI Gene Symbol: ENPP2
Gene Source: Human
Accession #: Q13822
Recombinant: Yes
Source: HEK293 cells
Purity by SDS-PAGE #: ≥92%
Assay: SDS-PAGE
Purity: N/A
Assay #2: N/A
Endotoxin Level: <1 EU/μg by LAL method
Activity (Specifications/test method): N/A
Biological activity: Measured by its ability to cleave Bis (p-Nitrophenyl) Phosphate (BPNPP). The specific activity is >8000 pmol/min/µg.
Results: >8000 pmol/min/µg
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: This protein is fused with polyhistidine tag at the N-terminus, has a calculated MW of 94.5 kDa. The predicted N-terminus is Asp 49. DTT-reduced Protein migrates as 120-130 kDa due to glycosylation.
Concentration: N/A
Appearance: Lyophilized
Physical form description: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH 7.5. Normally Mannitol or Trehalose are added as protectants before lyophilization.
Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in PBS, pH 7.4. Do not vortex.
Background Information: Autotaxin also known as ectonucleotide pyrophosphatase / phosphodiesterase family member 2 (E-NPP 2 or ENPP2), Extracellular lysophospholipase D (LysoPLD), Autotaxin (ATX or ATX-X), which belongs to the nucleotide pyrophosphatase / phosphodiesterase family. ENPP2 contains two SMB (somatomedin-B) domains. ENPP2 hydrolyzes lysophospholipids to produce lysophosphatidic acid (LPA) in extracellular fluids. ENPP2 can act on sphingosylphosphphorylcholine producing sphingosine-1-phosphate, a modulator of cell motility. ENPP2 stimulates migration of melanoma cells, probably via a pertussis toxin-sensitive G protein.
Amino acid sequence: N/A
Handling: Centrifuge the vial prior to opening.
Usage: For Research Use Only! Not to be used in humans
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