Human CellExp™ EpCAM/CD326, human recombinant

Biomolecule/Target: EpCAM/CD326

Synonyms: EPCAM, DIAR5, DIAR-5, EGP-2, EGP2, EGP314, EGP-314, EGP40, EGP-40, ESA, GA733-2, HNPCC8, HNPCC-8, KS1/4, KSA, M4S1, MIC18, MIC-18, MK-1, MK1, TACSTD1, TACSTD-1, TROP1, TROP-1, CD326, CD-326, Epithelial cell adhesion molecule, tumor-associated calcium signal transducer 1, cluster of differentiation 326.

Alternates names: EPCAM, DIAR5, DIAR-5, EGP-2, EGP2, EGP314, EGP-314, EGP40, EGP-40, ESA, GA733-2, HNPCC8, HNPCC-8, KS1/4, KSA, M4S1, MIC18, MIC-18, MK-1, MK1, TACSTD1, TACSTD-1, TROP1, TROP-1, CD326, CD-326, Epithelial cell adhesion molecule, tumor-associated calcium signal transducer 1, cluster of differentiation 326.

Taglines: A pan-epithelial differentiation antigen that is expressed on almost all carcinomas

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 4072

NCBI Gene Symbol: EPCAM

Gene Source: Human

Accession #: P16422

Recombinant: Yes

Source: HEK293 cells

Purity by SDS-PAGE #: ≥95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: <1 EU/μg by LAL method

Activity (Specifications/test method): N/A

Biological activity: Measured by its binding ability in a functional ELISA. Immobilized recombinant human Cathepsin V at 10 μg/mL (100 μl/well) can bind biotinylated EpCAM. The EC50 of biotinylated EpCAM is 50-70 ng/mL.

Results: Measured by its binding ability in a functional ELISA. Immobilized recombinant human Cathepsin V at 10 μg/mL (100 μl/well) can bind biotinylated EpCAM. The EC50 of biotinylated EpCAM is 50-70 ng/mL.

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: This protein contains a poly histidine tag at C-terminus and has a calculated MW of 29 kDa after removal of signal peptide. The predicted N-terminus is Gln24. In DTT-reduced SDS-PAGE, rh EpCAM protein migrates as 33-36 kDa poly peptide due to glycosylation.

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Background Information: EpCAM is also known as CO171A,; EGP; EGP40; GA7332; KSA; M4S; MIC18; MK1; TROP1; hEGP2, and is a pan-epithelial differentiation antigen that is expressed on almost all carcinomas as 17-1A(mAb) antigen. Its constitutional function is being elucidated. It is intricately linked with the Cadherin-Catenin pathway and hence the fundamental WNT pathway responsible for intracellular signaling and polarity. The epithelial cell adhesion molecule (Ep-CAM) is known to express in most epithelial malignancies and was reported as a tumor marker or a candidate of molecular targeting therapy. Ep-CAM cross signaling with N-cadherin involves Pi3K, resulting in the abrogation of the cadherin adhesion complexes in epithelial cells was reported. And epithelial cell adhesion molecule (Ep-CAM) recently received increased attention as a prognostic factor in breast cancer.

Amino acid sequence: N/A

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans