Human CellExp™ Fetuin A/ASHG, Human Recombinant

Biomolecule/Target: Fetuin A

Synonyms: Alpha-2-HS-glycoprotein, Alpha-2-Z-globulin, Ba-alpha-2-glycoprotein

Alternates names: Alpha-2-HS-glycoprotein, Alpha-2-Z-globulin, Ba-alpha-2-glycoprotein

Taglines: Exerts various calcium dependent physiological activities

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 197

NCBI Gene Symbol: FETUA

Gene Source: Human

Accession #: P02765

Recombinant: Yes

Source: HEK 293 cells

Purity by SDS-PAGE #: ≥95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: < 0.1 ng/μg of protein (<1EU/μg).

Activity (Specifications/test method): Determined by its ability to inhibit Cathepsin V cleavage of a fluorogenic peptide substrate ZLR-AMC (RLUs). The expected IC50 is ≤ 100nM.

Biological activity: Determined by its ability to inhibit Cathepsin V cleavage of a fluorogenic peptide substrate ZLR-AMC (RLUs). The expected IC50 is ≤ 100nM.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 45-55 kDa

Concentration: N/A

Appearance: Lyophilized powder

Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM Sodium Phosphate, pH 7.8.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Background Information: Fetuin A/AHSG is a human plasma glycoprotein belonging to the Cystatin family of proteases inhibitors. It is highly expressed in embryonic cells and adult hepatocytes, and is expressed to a lesser extent in monocytes/macrophages. Fetuin A/AHSG is a major serum protein component that exerts various calcium dependent physiological activities, and can mediate growth signaling in certain tumor cells. It also can act as a natural antagonist against specific TGF-β and BMP signaling proteins. Recombinant human Fetuin-A is a heterodimeric protein containing a 282 amino acid A chain and a 27 amino acid B chain. Due to glycosylation, recombinant Fetuin-A migrates at an apparent molecular weight of 45 – 55 kDa by SDS-PAGE run under non-reducing conditions.

Amino acid sequence: A-Chain: APHGPGLIYR QPNCDDPETE EAALVAIDYI NQNLPWGYKH TLNQIDEVKV WPQQPSGELF EIEIDTLETT CHVLDPTPVA RCSVRQLKEH AVEGDCDFQL LKLDGKFSVV YAKCDSSPDS AEDVRKVCQD CPLLAPLNDT RVVHAAKAAL AAFNAQNNGS NFQLEEISRA QLVPLPPSTY VEFTVSGTDC VAKEATEAAK CNLLAEKQYG FCKATLSEKL GGAEVAVTCT VFQTQPVTSQ PQPEGANEAV PTPVVDPDAP PSPPLGAPGL PPAGSPPDSH VL B-Chain: TVVQPSVGAA AGPVVPPCPG RIRHFKV

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans