Human CellExp™ FGL1, Human Recombinant

Biomolecule/Target: N/A

Synonyms: FGL1, Hepassocin, HP-041, HFREP-1, LFIRE-1, HFREP1

Alternates names: FGL1, Hepassocin, HP-041, HFREP-1, LFIRE-1, HFREP1

Taglines: A novel targets for radiation-induced lung fibrosis

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 2267

NCBI Gene Symbol: FGL1

Gene Source: Human

Accession #: Q08830

Recombinant: True

Source: HEK 293 cells

Purity by SDS-PAGE #: >95%

Assay: N/A

Purity: N/A

Assay #2: N/A

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Activity (Specifications/test method): Immobilized Human LAG-3, Mouse IgG2a Fc Tag at 10 μg/mL (100 μL/well) can bind Human FGL1, His Tag with a linear range of 0.078-0.625 μg/mL. Serial dilutions of Anti-LAG3, Neutralizing Antibody were added into Human FGL1, His Tag: Biotinylated Human LAG-3, Fc, Avitag binding reactions. The half maximal inhibitory concentration (IC50) is 0.16695 μg/mL

Biological activity: N/A

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 36.0 kDa. The protein migrates as 33-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Concentration: N/A

Appearance: Lyophilized powder

Physical form description: Lyophilized

Reconstitution Instructions: Reconstitute in sterile deionized water to the desired protein concentration.

Background Information: Fibrinogen-like protein 1(FGL1) is also known as HP-041, Hepassocin, HFREP-1, LFIRE-1. The protective effect of fibrinogen-like protein 1 (FGL1) in liver injury has previously been reported. However, studies have shown that FGL1 may be a predictor of GC patients and a target for GC therapy. Immunocytochemical studies revealed that fgl1 selectively binds to defective spermatozoa in the cauda epididymidis. Northern blot analysis and in situ hybridization demonstrated the high expression of fgl1 in the principal cells of the proximal cauda epididymidis. Immunofluorescence analysis using mouse fibrotic lung tissues suggested that fibrotic regions showed increased expressions of Gtse1 and Fgl1, Gtse1 and Fgl1 are suggested to be novel targets for radiation-induced lung fibrosis.

Amino acid sequence: Leu 23 - Ile 312

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans