Human CellExp™ FOLR1, mouse recombinant
- Usually Shipped in 5 Working Days
- Storage Temperature:
- Shipping Conditions:
- Gel Pack
- Shelf Life:
- 12 months
Synonyms: FOLR1, FBP, FOLR, FOLR-1, Folate-receptor-alpha
Alternates names: FOLR1, FBP, FOLR, FOLR-1, Folate-receptor-alpha
Taglines: Mediates delivery of 5-methyltetrahydrofolate to the interior of cells
Country of Animal Origin: USA
NCBI Gene ID #.: 14275
NCBI Gene Symbol: FOLR1
Gene Source: Mouse
Accession #: P35846
Source: HEK293 cells
Purity by SDS-PAGE #: ≥95%
Assay #2: N/A
Endotoxin Level: <1 EU/μg by LAL method
Activity (Specifications/test method): N/A
Biological activity: Measured in a competitive binding assay. When Folic Acid-Bovine Serum Albumin is immobilized at 5 μg/mL (100 μL/well), Recombinant Mouse (rm) FOLR1 inhibits 50% binding of biotinylated rm FOLR1 (7.9 nM) at the concentration range of 10-100 nM.
Results: Measured in a competitive binding assay. When Folic Acid-Bovine Serum Albumin is immobilized at 5 μg/mL (100 μL/well), Recombinant Mouse (rm) FOLR1 inhibits 50% binding of biotinylated rm FOLR1 (7.9 nM) at the concentration range of 10-100 nM.
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: This protein is fused with 6×his tag at the C-terminus and has a calculated MW of 25.6 kDa expressed. The predicted N-terminus is Thr 25. Protein migrates as 45-50 kDa in reduced SDS-PAGE resulting from glycosylation.
Physical form description: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.
Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Background Information: Folate Receptor 1 (FOLR1) also known as Folate receptor alpha, Folate Binding Protein (FBP), FOLR, and is a member of the folate receptor (FOLR) family. Members of this gene family have a high affinity for folic acid and for several reduced folic acid derivatives, and mediate delivery of 5-methyltetrahydrofolate to the interior of cells. Mature FOLR1 is an N-glycosylated protein that is anchored to the cell surface by a GPI linkage. FOLR1 is predominantly expressed on epithelial cells and is dramatically upregulated on many carcinomas. FOLR1 is internalized to the endosomal system where it dissociates from its ligand before recycling to the cell surface. A soluble form of FOLR1 can be proteolytically shed from the cell surface into the serum and breast milk. Defects in FOLR1 are the cause of neurodegeneration due to cerebral folate transport deficiency (NCFTD). NCFTD is an autosomal recessive disorder resulting from brain-specific folate deficiency early in life.
Amino acid sequence: N/A
Handling: Centrifuge the vial prior to opening.
Usage: For Research Use Only! Not to be used in humans