Human CellExp™ FOLR1, Fc Tag, Human Recombinant

Biomolecule/Target: FOLR1

Synonyms: FOLR1, FBP, FOLR, FOLR-1, Folate-receptor-alpha

Alternates names: FOLR1, FBP, FOLR, FOLR-1, Folate-receptor-alpha

Taglines: Mediates delivery of 5-methyltetrahydrofolate to the interior of cells

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 2348

NCBI Gene Symbol: FOLR1

Gene Source: Human

Accession #: P15328

Recombinant: Yes

Source: HEK293 cells

Purity by SDS-PAGE #: ≥95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: < 1.0 EU per/μg

Activity (Specifications/test method): Measured by its binding ability in a functional ELISA.

Biological activity: Immobilized Folic acid-BSA conjugate at 5 μg/mL (100 μL/well) can bind Human FOLR1, Fc Tag with a linear range of 10-78 ng/mL

Results: N/A

Binding Capacity: Immobilized Folic acid-BSA conjugate at 5 μg/mL (100 μL/well) can bind Human FOLR1, Fc Tag with a linear range of 10-78 ng/mL

Unit Definition: N/A

Molecular Weight: 51.3 kDa

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.

Reconstitution Instructions: Reconstitute in sterile deionized water to a concentration of 50 μg/ml.

Background Information: Folate Receptor 1 (FOLR1) also known as Folate receptor alpha, Folate Binding Protein (FBP), FOLR, and is a member of the folate receptor (FOLR) family. Members of this gene family have a high affinity for folic acid and for several reduced folic acid derivatives, and mediate delivery of 5-methyltetrahydrofolate to the interior of cells. Mature FOLR1 is an N-glycosylated protein that is anchored to the cell surface by a GPI linkage. FOLR1 is predominantly expressed on epithelial cells and is dramatically upregulated on many carcinomas. FOLR1 is internalized to the endosomal system where it dissociates from its ligand before recycling to the cell surface. A soluble form of FOLR1 can be proteolytically shed from the cell surface into the serum and breast milk. Defects in FOLR1 are the cause of neurodegeneration due to cerebral folate transport deficiency (NCFTD). NCFTD is an autosomal recessive disorder resulting from brain-specific folate deficiency early in life.

Amino acid sequence: N/A

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans