Human CellExp™ IGF1R/CD221, human recombinant

Biomolecule/Target: IGF1R/CD221

Synonyms: IGF-1R, IGF1R, CD221, IGFIR, IGFⅠR, IGFR, JTK13, MGC142170, MGC142172, MGC18216, Insulin-like Growth Factor 1 (IGF-1) Receptor

Alternates names: IGF-1R, IGF1R, CD221, IGFIR, IGFⅠR, IGFR, JTK13, MGC142170, MGC142172, MGC18216, Insulin-like Growth Factor 1 (IGF-1) Receptor

Taglines: This receptor mediates the effects of IGF-1

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 3480

NCBI Gene Symbol: IGF1R

Gene Source: Human

Accession #: P08069

Recombinant: Yes

Source: HEK293 cells

Purity by SDS-PAGE #: ≥95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: <1 EU/μg by LAL method

Activity (Specifications/test method): N/A

Biological activity: Measured by its ability to bind human IGF-I in a functional ELISA.

Results: Measured by its ability to bind human IGF-I in a functional ELISA.

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: This protein contains C-terminal polyhistidine tag and has a calculated MW of 104 kDa (single chain), 80 kDa (α subunit) and 23 kDa (β subunit). DTT-reduced protein migrates as 120 kDa, 80 kDa and 35 kDa polypeptide in SDS-PAGE.

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Background Information: The Insulin-like Growth Factor 1 Receptor （IGF1R）is also known as CD221, JTK13, and is a transmembrane receptor that is activated by IGF-1 and by the related growth factor IGF-2. It belongs to the large class of tyrosine kinase receptors. This receptor mediates the effects of IGF-1, which is a polypeptide protein hormone similar in molecular structure to insulin. IGF1R is made up of two alpha subunits and two beta subunits. Both the α and β subunits are synthesized from a single mRNA precursor. The precursor is then glycosylated, proteolytically cleaved, and crosslinked by cysteine bonds to form a functional transmembrane αβ chain. The α chains are located extracellularly while the β subunit spans the membrane and are responsible for intracellular signal transduction upon ligand stimulation. IGF1R have a binding site for ATP, which is used to provide the phosphates for autophosphorylation. There is a 60% homology between IGF1R and the insulin receptor. In response to ligand binding, the α chains induce the tyrosine autophosphorylation of the β chains. This event triggers a cascade of intracellular signaling that, while somewhat cell type specific, often promotes cell survival and cell proliferation.

Amino acid sequence: N/A

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans