Human CellExp™ IL-1 RII /CD121b, human recombinant

Biomolecule/Target: IL-1 RII /CD121b

Synonyms: CD121b, IL1RB, IL1R2, CDw121b

Alternates names: CD121b, IL1RB, IL1R2, CDw121b

Taglines: Serves as a decoy receptor by competitive binding to IL1B

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 7850

NCBI Gene Symbol: IL1R2

Gene Source: Human

Accession #: P27930

Recombinant: Yes

Source: HEK293 cells

Purity by SDS-PAGE #: ≥95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: <1 EU/μg by LAL method

Activity (Specifications/test method): N/A

Biological activity: Measured by its ability to inhibit IL-1 beta dependent proliferation in D10.G4.1 mouse helper T cells. Approximately 0.5-3 µg/ml of IL1R2 will inhibit 50% of the biological response due to 50 pg/ml of recombinant human IL-1 beta.

Results: Measured by its ability to inhibit IL-1 beta dependent proliferation in D10.G4.1 mouse helper T cells. Approximately 0.5-3 µg/ml of IL1R2 will inhibit 50% of the biological response due to 50 pg/ml of recombinant human IL-1 beta.

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: This protein is fused with 6×His tag at the C-terminus, has a calculated MW of 38.6 kDa. The predicted N-terminus is Thr 14. DTT-reduced Protein migrates as 50-55 kDa due to glycosylation.

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Background Information: Interleukin-1 receptor type 2 (IL1R2) also known as CD121 antigen-like family member B (CDw121b), IL-1 type II receptor, Interleukin-1 receptor type II, belongs to the interleukin-1 receptor family. Two distinct types of IL1 receptors which are able to bind IL1 specifically have been identified designated as IL1RI (IL1RA) and IL1RII (IL1RB). IL1R2 is non-signaling receptor for IL1A, IL1B and IL1RN, reduces IL1B activities. Serves as a decoy receptor by competitive binding to IL1B and preventing its binding to IL1R1. IL1R2 modulates cellular response through non-signaling association with IL1RAP after binding to IL1B. IL1R2 (membrane and secreted forms) preferentially binds IL1B and poorly IL1A and IL1RN. The secreted IL1R2 recruits secreted IL1RAP with high affinity; this complex formation may be the dominant mechanism for neutralization of IL1B by secreted/soluble receptors.

Amino acid sequence: N/A

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans