Human CellExp™ IL-17A, Human Recombinant

Biomolecule/Target: N/A

Synonyms: Human Cellexp Human Recombinant IL-17A

Alternates names: CTLA-8, IL-17, IL-17A, Cytotoxic T-lymphocyte-associated antigen 8.

Taglines: A proinflammatory cytokine produced by activated T cells

Taglines: USA

Country of Animal Origin: China

NCBI Gene ID #.: 3605

NCBI Gene Symbol: IL17A

Gene Source: Human

Accession #: Q16552

Recombinant: Yes

Source: Human 293 cell expressed

Purity by SDS-PAGE #: > 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: < 1.0 EU per 1 μg of protein

Activity (Specifications/test method): N/A

Biological activity: ED₅₀ is typically 5 to 25 ng/mL. The specific activity was determined by the dose-dependent induction of IL-6 secretion from NDHF Adult fibroblasts.

Results: 5 to 25 ng/ml

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 30 to 35 kDa, homodimer, glycosylated

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized in 10 mM Tris, pH 7.4 and 250 mM NaCl.

Reconstitution Instructions: Reconstitute in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.

Background Information: Interleukin-17 is a proinflammatory cytokine produced by activated T cells. IL-17 regulates the activities of NF-kappa B and mitogen-activated protein kinases. IL17 can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of IL-17 are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. The original described IL-17 protein, now known as IL-17A, was identified from a CD4+ T cells DNA library. IL-17 can be induced from primary peripheral blood CD4+ T cells upon stimulation. Supernatant from cells transfected with IL-17 induced IL-16 and IL-18 production and enhanced the surface expression of the intracellular adhesion molecule-1 (ICAM-1) in human fibroblasts.

Amino acid sequence: N/A

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans