Human CellExp™ IL-17E, human recombinant
- Usually Shipped in 5 Working Days
- 10 μg
- Storage Temperature:
- Shipping Conditions:
- Gel Pack
- Shelf Life:
- 12 months
Synonyms: IL-17E, IL-25, Interleukin-25
Alternates names: IL-17E, IL-25, Interleukin-25
Taglines: Induces NF-κB activation and stimulates the production of IL8
Country of Animal Origin: USA
NCBI Gene ID #.: 64806
NCBI Gene Symbol: IL-17E
Gene Source: Human
Accession #: Q9H293
Source: HEK 293 cells
Purity by SDS-PAGE #: ≥95%
Assay #2: HPLC
Endotoxin Level: < 0.1 ng/μg of protein (<1EU/μg).
Activity (Specifications/test method): The activity is determined by the dose-dependent production of IL-8 by human PBMCs. The expected ED50 for this effect is 12-25 ng/ml.
Biological activity: The activity is determined by the dose-dependent production of IL-8 by human PBMCs. The expected ED50 for this effect is 12-25 ng/ml.
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: This protein is fused with 6 × His tag at the C-terminus, has a calculated MW of 16.7 kDa. The predicted N-terminus is Tyr 33. DTT-reduced Protein migrates as 24 kDa, 27 kDa and 31 kDa due to different glycosylation.
Appearance: Lyophilized powder
Physical form description: Lyophilized from 0.22 μm filtered solution in PBS. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.
Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Background Information: Interleukin 25 (IL25) also known as Interleukin-17E (IL-17E), is a distinct member of the IL17 cytokine family comprised of at least six members sharing a conserved cysteine-knot structure but divergent at the N-terminus. IL-25 is secreted by type 2 helper T cells (Th2) and mast cells. IL25 can induce NF-κB activation, and stimulate the production of IL8. Both this cytokine and IL17B are ligands for the cytokine receptor IL17RB. IL-25 induces the production of other cytokines, including IL-4, IL-5 and IL-13 in multiple tissues, which stimulate the expansion of eosinophils. This cytokine is an important molecule controlling immunity of the gut and has been implicated in chronic inflammation associated with the gastrointestinal tract. IL-25 can kill some types of breast cancer cells.
Amino acid sequence: N/A
Handling: Centrifuge the vial prior to opening.
Usage: For Research Use Only! Not to be used in humans