Human CellExp™ IL7Ra/CD127, human recombinant

Biomolecule/Target: IL7Ra/CD127

Synonyms: IL7Ra, CD127

Alternates names: IL7Ra, CD127

Taglines: A receptor for IL-7 and TSLP

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 23765

NCBI Gene Symbol: IL17Ra

Gene Source: Human

Accession #: Q96F46

Recombinant: Yes

Source: HEK293 cells

Purity by SDS-PAGE #: ≥95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: <1 EU/μg by LAL method

Activity (Specifications/test method): N/A

Biological activity: Measured by its binding ability in a functional ELISA. Immobilized rhIL7Ra Fc Chimera at 0.25 μg/ml (100 μl/well) on a Rabbit anti‑human IgG Fc antibody, the concentration of rhIL-7 that produces 50% of the optimal binding response is found to be approximately 20 - 100 ng/mL.

Results: Measured by its binding ability in a functional ELISA. Immobilized rhIL7Ra Fc Chimera at 0.25 μg/ml (100 μl/well) on a Rabbit anti‑human IgG Fc antibody, the concentration of rhIL-7 that produces 50% of the optimal binding response is found to be approximately 20 - 100 ng/mL.

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: This protein is fused with Fc fragment of human IgG1 at the C-terminus, has a calculated MW of 51 kDa. The predicted N-terminus is Glu 21. DTT-reduced Protein migrates as 66-90 kDa due to glycosylation.

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Background Information: Interleukin-7 receptor subunit alpha (IL7Ra) also known as CD antigen CD127, which belongs to the type I cytokine receptor family and type 4 subfamily. IL7Ra /CD127 contains 1 fibronectin type-III domain. IL7Ra /CD127 is expressed on various cell types, including naive and memory T cells and many others. IL7Ra /CD127 is receptor for interleukin-7 and also acts as a receptor for thymic stromal lymphopoietin (TSLP). The IL7 receptor is a heterodimer of IL7R and IL2RG. The TSLP receptor is a heterodimer of CRLF2 and IL7R.

Amino acid sequence: N/A

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans