Biovision
Human CellExp™ LEPR/CD295, human recombinant
- SKU:
- 26-7497
- Availability:
- Usually Shipped in 5 Working Days
- Storage Temperature:
- -20°C
- Shipping Conditions:
- Gel Pack
- Shelf Life:
- 12 months
Description
Biomolecule/Target: LEPR/CD295
Synonyms: LEPR, CD295, DKFZp686B1731, LEP-R, OB-R, OBR, Leptin receptor
Alternates names: LEPR, CD295, DKFZp686B1731, LEP-R, OB-R, OBR, Leptin receptor
Taglines: Mediates the biological function of the adipocyte-specific hormone Leptin
Taglines: USA
Country of Animal Origin: USA
NCBI Gene ID #.: 3953
NCBI Gene Symbol: LEPR
Gene Source: Human
Accession #: P48357
Recombinant: Yes
Source: HEK293 cells
Purity by SDS-PAGE #: ≥95%
Assay: SDS-PAGE
Purity: N/A
Assay #2: N/A
Endotoxin Level: <1 EU/μg by LAL method
Activity (Specifications/test method): N/A
Biological activity: Measured by its ability to inhibit Leptin-dependent proliferation of BaF3 mouse pro-B cells transfected with rhLEPR-Fc. The ED50 for this effect is typically 0.015-0.13 μg/ml in the presence of 3 ng/ml rhLeptin.
Results: Measured by its ability to inhibit Leptin-dependent proliferation of BaF3 mouse pro-B cells transfected with rhLEPR-Fc. The ED50 for this effect is typically 0.015-0.13 μg/ml in the presence of 3 ng/ml rhLeptin.
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: This protein is fused with Fc fragment of human IgG1 at the C-terminus and has a calculated MW of 121 kDa expressed. The predicted N-terminus is Phe22. Protein migrates as 150-165 kDa in reduced SDS-PAGE resulting from glycosylation.
Concentration: N/A
Appearance: Lyophilized
Physical form description: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.
Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Background Information: Leptin receptor (LEPR), also known as LEP-R, cluster of differentiation 295 (CD295), OB-R and B219, is a single-transmembrane-domain receptor of the gp130 family of cytokine receptors. Leptin receptor exists as homodimer and binds Leptin with high affinity, thus mediates the biological function of the adipocyte-specific hormone Leptin. LEPR is a receptor for leptin (an adipocyte-specific hormone that regulates body weight), and is involved in the regulation of fat metabolism, as well as in a novel hematopoietic pathway that is required for normal lymphopoiesis. Mutations in this protein have been associated with obesity and pituitary dysfunction. Interaction of leptin and leptin receptor is crucial for body weight and bone mass regulation in mammals through hypothalamic effects on satiety and energy expenditure. Meanwhile, research data supports a leptin receptor activation model based on ligand-induced conformational changes.
Amino acid sequence: N/A
Handling: Centrifuge the vial prior to opening.
Usage: For Research Use Only! Not to be used in humans