Human CellExp™ MBL/MBL2, human recombinant

Biomolecule/Target: MBL/MBL2

Synonyms: MBL, MBL2, MBP1, MBP-C, Collectin-1, COLEC1, HSMBPC, MBL2D, MBPD, Mannose binding Lectin

Alternates names: MBL, MBL2, MBP1, MBP-C, Collectin-1, COLEC1, HSMBPC, MBL2D, MBPD, Mannose binding Lectin

Taglines: >95% Pure, active, Human cell expressed MBL/MBL2/Mannose binding Lectin

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 4153

NCBI Gene Symbol: MBL2

Gene Source: Human

Accession #: P11226

Recombinant: Yes

Source: HEK 293 cells

Purity by SDS-PAGE #: ≥95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: < 1.0 EU per μg of the protein by the LAL method.

Activity (Specifications/test method): Measured by its ability to bind with Mannan in a functional ELISA.

Biological activity: Measured by its ability to bind with Mannan in a functional ELISA.

Results: Measured by its ability to bind with Mannan in a functional ELISA.

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: This protein is fused with 6×His tag at the C-terminus, has a calculated MW of 24.8 kDa. The predicted N-terminus is Glu 21. DTT-reduced Protein migrates as 30-33 kDa.

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in PBS, pH 7.4. Do not vortex.

Background Information: Mannose-binding lectin (MBL) is also known as mannose-binding protein, mannan-binding protein (MBP), Mannose-binding protein C, Collectin-1 (COLEC1), MBL2, which belongs to the class of collectins in the C-type lectin superfamily. MBL contains one C-type lectin domain and one collagen-like domain. MBL has an oligomeric structure (400-700 kDa), built of subunits that contain three presumably identical peptide chains of about 30 kDa each. MBL is calcium-dependent lectin involved in innate immune defense. MBL binds mannose, fucose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. MBL binds to late apoptotic cells. MBL may bind DNA.

Amino acid sequence: N/A

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans