Human CellExp™ Mesothelin / MSLN, Human Recombinant

Biomolecule/Target: Mesothelin

Synonyms: MSLN, Mesothelin, MPF, CAK1 antigen, Pre-pro-megakaryocyte-potentiating factor

Alternates names: MSLN, Mesothelin, MPF, CAK1 antigen, Pre-pro-megakaryocyte-potentiating factor

Taglines: Membrane-anchored forms may play a role in cellular adhesion.

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 10232

NCBI Gene Symbol: MSLN

Gene Source: Human

Accession #: Q13421

Recombinant: Yes

Source: HEK293 cells

Purity by SDS-PAGE #: ≥95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: <1 EU/μg by LAL method

Activity (Specifications/test method): Measured by its binding ability in a functional ELISA.

Biological activity: Immobilized Human Mesothelin, His Tag at 0.5 μg/mL (100 μL/well) can bind Anti-Human MSLN Mab with a linear range of 0.1-2 ng/mL

Results: N/A

Binding Capacity: Immobilized Human Mesothelin, His Tag at 0.5 μg/mL (100 μL/well) can bind Anti-Human MSLN Mab with a linear range of 0.1-2 ng/mL

Unit Definition: N/A

Molecular Weight: 33.0 kDa

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Reconstitution Instructions: Reconstitute in sterile deionized water to a concentration of 50 μg/ml.

Background Information: Mesothelin (MSLN) is also known as CAK1 antigen, Pre-pro-megakaryocyte-potentiating factor, which belongs to the mesothelin family. Mesothelin / MSLN can be proteolytically cleaved into the following two chains by a furin-like convertase: Megakaryocyte-potentiating factor (MPF) and the cleaved form of mesothelin. Both MPF and the cleaved form of mesothelin are N-glycosylated. Mesothelin / MSLN can interacts with MUC16. The membrane-anchored forms of MSLN may play a role in cellular adhesion. MPF potentiates megakaryocyte colony formation in vitro.

Amino acid sequence: N/A

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans