Human CellExp™ MSR1/CD204, human recombinant

Biomolecule/Target: MSR1/CD204

Synonyms: MSR1, CD204, SCARA1, SR-A, phSR1, phSR2

Alternates names: MSR1, CD204, SCARA1, SR-A, phSR1, phSR2

Taglines: Mediates the endocytosis of modified low density lipoproteins (LDLs)

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 4481

NCBI Gene Symbol: MSR1

Gene Source: Human

Accession #: P21757

Recombinant: Yes

Source: HEK293 cells

Purity by SDS-PAGE #: ≥95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: <1 EU/μg by LAL method

Activity (Specifications/test method): N/A

Biological activity: Measured by its binding ability in a functional ELISA. Immobilized rhMSR1 at 5 µg/ml (100 µl/well) can bind biotinylated advanced glycation end products of bovine serum albumin (AGE-BSA, Cat # 2221-10) with a linear range of 1 - 150 ng/ml.

Results: Measured by its binding ability in a functional ELISA. Immobilized rhMSR1 at 5 µg/ml (100 µl/well) can bind biotinylated advanced glycation end products of bovine serum albumin (AGE-BSA, Cat # 2221-10) with a linear range of 1 - 150 ng/ml.

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: This protein is fused with 6×His tag at the C-terminus, has a calculated MW of 42.1 kDa. The predicted N-terminus is Lys 77. DTT-reduced Protein migrates as 65-72 kDa due to glycosylation.

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Background Information: Macrophage scavenger receptor 1 (MSR1) also known as CD204, SCARA1, SR-A, and is class A macrophage scavenger receptors, which include three different types (1, 2, 3) generated by alternative splicing of this gene. These receptors or isoforms are trimeric integral membrane glycoproteins and have been implicated in many macrophage-associated physiological and pathological processes. The isoforms type 1 and type 2 are functional receptors and are able to mediate the endocytosis of modified low density lipoproteins (LDLs). The isoform type 3 does not internalize modified LDL (acetyl-LDL) despite having the domain shown to mediate this function in the types 1 and 2 isoforms. Defects in MSR1 may be a cause of prostate cancer (PC) or Barrett esophagus (BE).

Amino acid sequence: N/A

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans