Human CellExp™ Osteoactivin / GPNMB, human recombinant

Biomolecule/Target: Osteoactivin / GPNMB

Synonyms: GPNMB, HGFIN, NMB, Osteoactivin

Alternates names: GPNMB, HGFIN, NMB, Osteoactivin

Taglines: ≥95% pure, active, human cell expressed, human recombinant Osteoactivin / GPNMB - acts as a downstream mediator of BMP-2.

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 10457

NCBI Gene Symbol: GPNMB

Gene Source: Human

Accession #: Q96F58

Recombinant: Yes

Source: HEK293 cells

Purity by SDS-PAGE #: ≥95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: <1 EU/μg by LAL method

Activity (Specifications/test method): N/A

Biological activity: Measured by the ability of the immobilized protein to induce pro-MMP-9 secretion by NHLF human normal lung fibroblasts. The ED50 for this effect is typically 4 - 16 µg/ml.

Results: ED50 for this effect is typically 4 - 16 µg/ml

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: This protein is fused with 6×His tag at the C-terminus, has a calculated MW of 52.9 kDa. The predicted N-terminus is Ala 22. DTT-reduced Protein migrates as 85-100 kDa due to glycosylation.

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.5. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in PBS, pH 7.4. Do not vortex.

Background Information: Transmembrane glycoprotein NMB (GPNMB) is also known as Transmembrane glycoprotein HGFIN, DC-HIL and Osteoactivin (OA), which belongs to the PMEL/NMB family. GPNMB contains one PKD domain. GPNMB is a transmembrane glycoprotein that is up-regulated in various cancer cells, including in glioblastoma multiforme and is expressed in many melanoma cells, as well as in tissue macrophages. GPNMB protein acts as a downstream mediator of BMP-2 effects on osteoblast differentiation and function. GPNMB participates in bone mineralization, and functions as a negative regulator of inflammation in macrophages.

Amino acid sequence: N/A

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans