Human CellExp™ Osteoprotegerin / TNFRSF11B, human recombinant

Biomolecule/Target: Osteoprotegerin / TNFRSF11B

Synonyms: TNFRSF11B, OCIF, OPG, Osteoprotegerin

Alternates names: TNFRSF11B, OCIF, OPG, Osteoprotegerin

Taglines: ≥95% pure, active, human cell expressed, human recombinant Osteoprotegerin / TNFRSF11B - acts as decoy receptor for TNFSF11/RANKL

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 4982

NCBI Gene Symbol: TNFRSF11B

Gene Source: Human

Accession #: O00300

Recombinant: Yes

Source: HEK293 cells

Purity by SDS-PAGE #: ≥95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: <1 EU/μg by LAL method

Activity (Specifications/test method): N/A

Biological activity: Measured by its binding ability in a functional ELISA. Immobilized rh TNFRSF11B at 10 μg/ml (100 μl/well) can bind human TNFSF11 Fc Chimera with a linear ranger of 4-256 ng/ml.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: This protein is fused with 6×His tag at the C-terminus, has a calculated MW of 44.4 kDa. The predicted N-terminus is Glu 22. DTT-reduced Protein migrates as 55 kDa due to glycosylation.

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.5. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in PBS, pH 7.4. Do not vortex.

Background Information: Tumor necrosis factor receptor superfamily member 11B (TNFRSF11B) is also known as Osteoclastogenesis inhibitory factor (OCIF), Osteoprotegerin (OPG). TNFRSF11B is a secreted homodimer protein, which can interact with TNFSF10 and TNFSF11. TNFRSF11B acts as decoy receptor for TNFSF11/RANKL and thereby neutralizes its function in osteoclastogenesis. TNFRSF11B inhibits the activation of osteoclasts and promotes osteoclast apoptosis in vitro. Bone homeostasis seems to depend on the local ratio between TNFSF11 and TNFRSF11B. TNFSF10/TRAIL binding blocks the inhibition of osteoclastogenesis.

Amino acid sequence: N/A

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans