Human CellExp™ OX40 Ligand / TNFSF4, Human recombinant

Biomolecule/Target: TNFRSF4

Synonyms: OX40L,TNFSF4,CD252

Alternates names: OX40L,TNFSF4,CD252

Taglines: A costimulatory molecule implicated in long-term T-cell immunity, also cts as a receptor for human herpesvirus 6B/HHV-6B

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 7293

NCBI Gene Symbol: TNFRSF4

Gene Source: Human

Accession #: NP_003317

Recombinant: Yes

Source: HEK 293 cells

Purity by SDS-PAGE #: >95%

Assay: SDS-PAGE

Purity: >95%

Assay #2: N/A

Endotoxin Level: < 1 EU/μg

Activity (Specifications/test method): Measured by its binding ability in a functional ELISA.

Biological activity: Immobilized HumanOX40, His Tag at 2 μg/ml (100 μl/well) can bind Human OX40 Ligand, Fc Tag with a linear range of 2-15 ng/ml.

Results: N/A

Binding Capacity: Immobilized HumanOX40, His Tag at 2 μg/ml (100 μl/well) can bind Human OX40 Ligand, Fc Tag with a linear range of 2-15 ng/ml.

Unit Definition: N/A

Molecular Weight: This protein is fused with a Fc tag at C-terminus and has a calculated MW of 20 kDa.

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized

Reconstitution Instructions: Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/ml

Background Information: Tumor necrosis factor ligand superfamily member 4 (TNFSF4) is also known as glycoprotein Gp34, OX40 ligand (OX40L), TAX transcriptionally-activated glycoprotein 1 and CD252, which belongs to the tumor necrosis factor family. TNFSF4 is the ligand for CD134 and is expressed on such cells as DC2s (a subtype of dendritic cells) enabling amplification of Th2 cell differentiation. The interaction of TNFSF4-TNFSF4 is involved in the pathogenesis of multiple autoimmune and inflammatory diseases such as systemic lupus erythematosus (SLE), carotid artery disease and cancer. Furthermore, similar to other TNF superfamily members, membrane-bound OX40 Ligand (TNFSF4) exists as a homotrimer. Human TNFSF4 shares 46% amino acid sequence identity with its mouse counterpart.

Amino acid sequence: N/A

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans