Human CellExp™ OX40 Ligand / TNFSF4, Mouse Recombinant

Biomolecule/Target: TNFSF4

Synonyms: OX40L, TNFSF4, CD252, Glycoprotein Gp34, TXGP1

Alternates names: OX40L, TNFSF4, CD252, Glycoprotein Gp34, TXGP1

Taglines: A costimulatory molecule implicated in long-term T-cell immunity, also cts as a receptor for human herpesvirus 6B/HHV-6B

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 22164

NCBI Gene Symbol: Tnfsf4

Gene Source: Mouse

Accession #: P43488

Recombinant: Yes

Source: HEK 293 cells

Purity by SDS-PAGE #: > 90%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: < 1.0 EU per/μg

Activity (Specifications/test method): Measured by its binding ability in a functional ELISA.

Biological activity: Immobilized Mouse OX40, Fc Tag at 5 μg/mL (100 μL/well) can bind Mouse OX40 Ligand, His Tag with a linear range of 0.5-16 ng/mL

Results: N/A

Binding Capacity: Immobilized Mouse OX40, Fc Tag at 5 μg/mL (100 μL/well) can bind Mouse OX40 Ligand, His Tag with a linear range of 0.5-16 ng/mL

Unit Definition: N/A

Molecular Weight: 18.3 kDa

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Generally Mannitol or Trehalose is added as a protectant before lyophilization.

Reconstitution Instructions: Reconstitute in sterile deionized water to the desired protein concentration.

Background Information: Members of the dickkopf-related protein family (DKK-1, -2, -3, and -4) are secreted proteins with two cysteine-rich domains separated by a linker region. And DKK3 has been proposed as tumour suppressor gene and a marker for tumour blood vessels. DKK3 is the only DKK family member abundantly expressed in normal lung, but silenced by promoter hypermethylation in a large fraction of lung cancer cell lines and lung tumors. Downregulation of DKK3 was correlated with tumor progression and expression of nuclear beta-catenin in lung tumors. Ectopic expression of DKK3 in lung cancer cells with DKK3 hypermethylation induced apoptosis and inhibited TCF-4 activity as well as nuclear accumulation of beta-catenin and expression of TCF-4 targets c-Myc and cyclin D1. DKK3 modulates FGF and Activin/Nodal signaling to regulate mesoderm induction during early Xenopus development, was reported.

Amino acid sequence: N/A

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans