Biovision
Human CellExp™ PCSK9, murine recombinant
- SKU:
- 26-7266
- Availability:
- Usually Shipped in 5 Working Days
- Size:
- 20 μg
- Storage Temperature:
- -20°C
- Shipping Conditions:
- Gel Pack
- Shelf Life:
- 12 months
Description
Biomolecule/Target: PCSK9
Synonyms: PCSK9, FH3, HCHOLA3, LDLCQ1, NARC-1, NARC1, PC9, Proprotein convertase subtilisin/kexin type 9
Alternates names: PCSK9, FH3, HCHOLA3, LDLCQ1, NARC-1, NARC1, PC9, Proprotein convertase subtilisin/kexin type 9
Taglines: Plays a major regulatory role in cholesterol homeostasis
Taglines: USA
Country of Animal Origin: USA
NCBI Gene ID #.: 100102
NCBI Gene Symbol: PCSK9
Gene Source: Mouse
Accession #: Q80W65
Recombinant: Yes
Source: HEK 293 cells
Purity by SDS-PAGE #: ≥97%
Assay: SDS-PAGE
Purity: N/A
Assay #2: N/A
Endotoxin Level: < 0.1 ng/μg of protein (<1EU/μg) by LAL method
Activity (Specifications/test method): Measured by its binding ability in a functional ELISA Immobilized mouse PCSK9 at 10 μg/ml (100 μl/well) can bind biotinylated rhLDLR. The EC50 of biotinylated human LDLR is 0.1 μg/ml.
Biological activity: Measured by its binding ability in a functional ELISA Immobilized mouse PCSK9 at 10 μg/ml (100 μl/well) can bind biotinylated rhLDLR. The EC50 of biotinylated human LDLR is 0.1 μg/ml.
Results: N/A
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: This protein is fused with 6×His tag at the N-terminus, has a calculated MW of 72 kDa. The predicted N-terminus is Gln 35. DTT-reduced Protein migrates as 20 kDa and 64 kDa due to glycosylation.
Concentration: N/A
Appearance: Lyophilized powder
Physical form description: Sterile filtered through a 0.22 micron filter. Lyophilized from 1 x PBS, pH 7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.
Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 100 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Background Information: Proprotein convertase subtilisin/kexin type 9 (PCSK9), is an enzyme which in humans is encoded by the PCSK9 gene. This gene encodes a proprotein convertase belonging to the proteinase K subfamily of the secretory subtilase family. This protein plays a major regulatory role in cholesterol homeostasis. PCSK9 binds to the epidermal growth factor-like repeat A (EGF-A) domain of the low-density lipoprotein receptor (LDLR), inducing LDLR degradation. PCSK9 may also have a role in the differentiation of cortical neurons. Mutations in this gene have been associated with a rare form of autosomal dominant familial hypercholesterolemia (HCHOLA3).
Amino acid sequence: N/A
Handling: Centrifuge the vial prior to opening.
Usage: For Research Use Only! Not to be used in humans