Human CellExp™ PD-L1 /CD274 /B7-H1, mouse recombinant

Biomolecule/Target: PD-L1 /CD274 /B7-H1

Synonyms: PD-L1, CD274, B7-H1, PDCD1L1, PDCD1LG1

Alternates names: PD-L1, CD274, B7-H1, PDCD1L1, PDCD1LG1

Taglines: N/A

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 60533

NCBI Gene Symbol: CD274

Gene Source: Mouse

Accession #: Q9EP73

Recombinant: Yes

Source: HEK293 cells

Purity by SDS-PAGE #: ≥95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: <1 EU/μg by LAL method

Activity (Specifications/test method): N/A

Biological activity: Measured by its binding ability in a functional ELISA. Immobilized recombinant mouse PDL1 at 1 μg/ml (100 μl/well) can bind biotinylated Mouse PDCD1 / PD1 with a linear range of 16 - 500 ng/ml.

Results: Measured by its binding ability in a functional ELISA. Immobilized recombinant mouse PDL1 at 1 μg/ml (100 μl/well) can bind biotinylated Mouse PDCD1 / PD1 with a linear range of 16 - 500 ng/ml.

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: This protein is fused with 6×His tag at the C-terminus, has a calculated MW of 25.6 kDa. The predicted N-terminus is Phe 19. DTT-reduced Protein migrates as 40-50 kDa due to glycosylation.

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Background Information: Programmed cell death 1 ligand 1 (PD-L1) is also known as cluster of differentiation (CD274) or B7 homolog 1 (B7-H1), is a member of the growing B7 family of immune molecules and is involved in the regulation of cellular and humoral immune responses. B7-H1 is a cell surface immunoglobulin superfamily with two Ig-like domains within the extracellular region and a short cytoplasmic domain. PD-L1 is highly expressed in the heart, skeletal muscle, placenta and lung and weakly expressed in the thymus, spleen, kidney and liver. PD-L1 is expressed on activated T-cells, B-cells, dendritic cells, keratinocytes and monocytes. PD-L1 is up-regulated on T- and B-cells, dendritic cells, keratinocytes and monocytes after LPS and IFNG activation and up-regulated in B-cells activated by surface Ig cross-linking. PD-L1 involve in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner.

Amino acid sequence: N/A

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans