Human CellExp™ CD273 / B7-DC / PD-L2, human recombinant

Biomolecule/Target: CD273/B7-DC/PD-L2

Synonyms: PDL2, PD-L2, Butyrophilin B7-DC, CD273, PDCD1 ligand 2, PDCD1L2, PDCD1LG2

Alternates names: PDL2, PD-L2, Butyrophilin B7-DC, CD273, PDCD1 ligand 2, PDCD1L2, PDCD1LG2

Taglines: A protein involved in the costimulatory signal essential for T-cell proliferation.

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 80380

NCBI Gene Symbol: PDCD1LG2

Gene Source: Human

Accession #: Q9BQ51

Recombinant: Yes

Source: HEK293 cells

Purity by SDS-PAGE #: ≥95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: <1 EU/μg by LAL method

Activity (Specifications/test method): N/A

Biological activity: Measured by its binding ability in a functional ELISA. Immobilized rh B7-DC / PD-L2 / CD273 at 1 µg/mL (100 µl/well) can bind rhPDCD-1 with a linear range of 15 - 500 ng/ml.

Results: Measured by its binding ability in a functional ELISA. Immobilized rh B7-DC / PD-L2 / CD273 at 1 µg/mL (100 µl/well) can bind rhPDCD-1 with a linear range of 15 - 500 ng/ml.

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: This protein is fused with polyhistidine tag at the C-terminus, has a calculated MW of 23.4 kDa. The predicted N-terminus is Leu 20. DTT-reduced Protein migrates as 35-48 kDa due to glycosylation.

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Background Information: Programmed cell death 1 ligand 2 (PD-L2 or PDCD1 ligand 2) is also known as Butyrophilin B7-DC, CD antigen CD273, which belongs to the immunoglobulin superfamily or BTN/MOG family. The expression of PD-L2 is up-regulated by IFNG/IFN-gamma stimulation in monocytes and induced on dendritic cells grown from peripheral blood mononuclear cells with CSF2 and IL-4. PD-L2 Involved in the costimulatory signal, essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. PD-L2 interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production.

Amino acid sequence: N/A

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans