Human CellExp™ PDGF-BB, human recombinant
- Usually Shipped in 5 Working Days
- 10 μg
- Storage Temperature:
- Shipping Conditions:
- Gel Pack
- Shelf Life:
- 12 months
Synonyms: PDGFB, PDGF-B, FLJ12858, PDGF2, PDGF-2
Alternates names: N/A
Taglines: Mitogenic during early developmental stages
Country of Animal Origin: USA
NCBI Gene ID #.: 5155
NCBI Gene Symbol: PDGFB
Gene Source: Human
Accession #: P01127
Source: HEK 293 cells
Purity by SDS-PAGE #: ≥95%
Assay #2: N/A
Endotoxin Level: < 0.1 ng/μg of protein (<1EU/μg) by LAL method
Activity (Specifications/test method): The bio-activity was determined by dose-dependent stimulation of the proliferation of mouse 3T3 cells. ED50 was 1-10 ng/ml
Biological activity: The bio-activity was determined by dose-dependent stimulation of the proliferation of mouse 3T3 cells. ED50 was 1-10 ng/ml
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: This protein has a calculated MW of 25 kDa. DTT-reduced protein migrates as a 15 kDa polypeptide and the non-reduced cystine-linked homodimer migrates as a 30 kDa protein.
Appearance: Lyophilized powder
Physical form description: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl pH 8.0. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.
Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Background Information: N/A
Amino acid sequence: N/A
Handling: Centrifuge the vial prior to opening.
Usage: For Research Use Only! Not to be used in humans